Bovine DNA Damage Inducible Transcript 3 ELISA Kit | DDIT3 elisa kit

Bovine DNA Damage Inducible Transcript 3 ELISA Kit

Cat. Num. AAA746415

• Gene Names: DDIT3; CHOP; CEBPZ; CHOP10; CHOP-10; GADD153
• Reactivity: Bovine
• Synonyms: DNA Damage Inducible Transcript 3; Bovine DNA Damage Inducible Transcript 3 ELISA Kit; DDIT3 elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Intended Uses: This DDIT3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine DDIT3. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This DDIT3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine DDIT3. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DDIT3 elisa kit

Principle of the Assay: DDIT3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for DDIT3. Standards or samples are then added to the microtiter plate wells and DDIT3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of DDIT3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for DDIT3 are added to each well to "sandwich" the DDIT3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain DDIT3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DDIT3 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for DDIT3 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 304282234
• NCBI GeneID: 1649
• NCBI Accession #: NP_001181986.1
• NCBI GenBank Nucleotide #: NM_001195057.1
• Molecular Weight: 21,700 Da
• NCBI Official Full Name: DNA damage-inducible transcript 3 protein isoform 2
• NCBI Official Synonym Full Names: DNA-damage-inducible transcript 3
• NCBI Official Symbol: DDIT3
• NCBI Official Synonym Symbols: CHOP; CEBPZ; CHOP10; CHOP-10; GADD153
• NCBI Protein Information: DNA damage-inducible transcript 3 protein; DDIT-3; C/EBP zeta; c/EBP-homologous protein 10; CCAAT/enhancer-binding protein homologous protein; growth arrest and DNA damage-inducible protein GADD153
• UniProt Protein Name: DNA damage-inducible transcript 3 protein
• Protein Family: DNA damage-inducible transcript 3 protein
• UniProt Gene Name: DDIT3
• UniProt Synonym Gene Names: CHOP; CHOP10; GADD153; DDIT-3; CHOP; CHOP-10
• UniProt Entry Name: DDIT3_HUMAN

NCBI Description

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]

Uniprot Description

CHOP: a transcriptional-regulatory protein of the bZIP family. Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. May play an important role in melanoma progression. CK2-mediated phosphorylation inhibits its transcriptional activity. Up-regulates IL-6 transcription by trapping negative regulating NF-IL6 isoform.

Protein type: Oncoprotein; Transcription factor; DNA-binding; Autophagy

Chromosomal Location of Human Ortholog: 12q13.1-q13.2

Cellular Component: nucleoplasm; late endosome; cytosol; nucleus

Molecular Function: protein binding; leucine zipper domain binding; DNA binding; protein heterodimerization activity; transcription factor binding; transcription factor activity; transcription corepressor activity

Biological Process: regulation of transcription in response to stress; proteasomal ubiquitin-dependent protein catabolic process; negative regulation of protein kinase B signaling cascade; Wnt receptor signaling pathway; unfolded protein response; positive regulation of transcription, DNA-dependent; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; blood vessel maturation; mRNA transcription from RNA polymerase II promoter; response to unfolded protein; positive regulation of interleukin-8 production; response to starvation; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; regulation of transcription, DNA-dependent; cell redox homeostasis; positive regulation of neuron apoptosis; release of sequestered calcium ion into cytosol; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of myoblast differentiation; cell cycle arrest; inhibition of CREB transcription factor; response to DNA damage stimulus; ER overload response

Disease: Myxoid Liposarcoma