Rabbit anti-Human NDUFA12 Polyclonal Antibody | anti-NDUFA12 antibody

NDUFA12, CT (NDUFA12, DAP13, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12, 13kD differentiation-associated protein, Complex I-B17.2, NADH-ubiquinone oxidoreductase subunit B17.2) (PE)

Cat. Num. AAA6324374

• Gene Names: NDUFA12; B17.2; DAP13; MC1DN23
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NDUFA12; Polyclonal Antibody; CT (NDUFA12; DAP13; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12; 13kD differentiation-associated protein; Complex I-B17.2; NADH-ubiquinone oxidoreductase subunit B17.2) (PE); NADH-ubiquinone oxidoreductase subunit B17.2; anti-NDUFA12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 145

• Applicable Applications for anti-NDUFA12 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NDUFA12 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 104-133 amino acids selected from the C-terminal region of human NDUFA12.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NDUFA12 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the NDUFA12 antibody detected the NDUFA12 protein (arrow).)

Related Product Information for anti-NDUFA12 antibody

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Product Categories/Family for anti-NDUFA12 antibody

Antibodies; Enzymes; Dehydrogenase

References

Murray, J., et al. J. Biol. Chem. 278(39):37223-37230(2003). Murray, J., et al. J. Biol. Chem. 278(39):37223-37230(2003). Murray, J., et al. J. Biol. Chem. 278(16):13619-13622(2003). Hu, R.M., et al. Proc. Natl. Acad. Sci. U.S.A. 97(17):9543-9548(2000). Triepels, R., et al. Hum. Genet. 106(4):385-391(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 10092657
• NCBI GeneID: 55967
• NCBI Accession #: NP_061326.1
• NCBI GenBank Nucleotide #: NP_061326.1
• UniProt Accession #: Q9UI09
• NCBI Official Full Name: NADH dehydrogenase
• NCBI Official Synonym Full Names: NADH:ubiquinone oxidoreductase subunit A12
• NCBI Official Symbol: NDUFA12
• NCBI Official Synonym Symbols: B17.2; DAP13; MC1DN23
• NCBI Protein Information: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
• UniProt Protein Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
• Protein Family: NADH dehydrogenase
• UniProt Gene Name: NDUFA12
• UniProt Synonym Gene Names: DAP13; CI-B17.2; CIB17.2
• UniProt Entry Name: NDUAC_HUMAN

NCBI Description

This gene encodes a protein which is part of mitochondrial complex 1, part of the oxidative phosphorylation system in mitochondria. Complex 1 transfers electrons to ubiquinone from NADH which establishes a proton gradient for the generation of ATP. Mutations in this gene are associated with Leigh syndrome due to mitochondrial complex 1 deficiency. Pseudogenes of this gene are located on chromosomes 5 and 13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012]