DBT recombinant protein

DBT Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8122107

• Gene Names: DBT; E2; E2B; BCATE2; BCKADE2; BCKAD-E2; BCOADC-E2
• Purity: >95% as determined by SDS-PAGE
• Synonyms: DBT; DBT Protein; Human; Recombinant (His Tag); Human DBT Protein (His Tag); BCATE2 Protein; BCKAD-E2 Protein; BCKADE2 Protein; E2 Protein; E2B Protein; RP11-305E17.3 Protein; DBT recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, pH8.0, 200mM NaCl, 20% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gly62-Lys482

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human DBT (NP_001909.3)(Gly62-Lys482) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 392494079
• NCBI GeneID: 1629
• NCBI Accession #: NP_001909.3
• NCBI GenBank Nucleotide #: NM_001918.3
• Molecular Weight: 53,487 Da
• NCBI Official Full Name: lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
• NCBI Official Synonym Full Names: dihydrolipoamide branched chain transacylase E2
• NCBI Official Symbol: DBT
• NCBI Official Synonym Symbols: E2; E2B; BCATE2; BCKADE2; BCKAD-E2; BCOADC-E2
• NCBI Protein Information: lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial

NCBI Description

The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]