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Western Blot (WB) (Western BlotPositive WB detected in: 293 whole cell lysate, Mouse kidney tissue, Mouse liver tissueAll lanes: DBT antibody at 4.3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 54 kDaObserved band size: 54 kDa)

Rabbit anti-Human, Mouse DBT Polyclonal Antibody | anti-DBT antibody

DBT Antibody

Gene Names
DBT; E2; E2B; BCATE2; BCKADE2; BCKAD-E2; BCOADC-E2
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
>95%
Protein G Purified
Synonyms
DBT; Polyclonal Antibody; DBT Antibody; Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; mitochondrial; EC 2.3.1.168; 52 kDa mitochondrial autoantigen of primary biliary cirrhosis; Branched chain 2-oxo-acid dehydrogenase complex component E2; BCOADC-E2; Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue; 2-methylpropanoyl)transferase; BCATE2; anti-DBT antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%
Protein G Purified
Form/Format
Liquid
Sequence Length
482
Applicable Applications for anti-DBT antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 1:500-1:5000
Immunogen Species
Human
Immunogen
Recombinant Human Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial protein (140-259AA)
Conjugate
Non-Conjugated
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at-20 degree C or-80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: 293 whole cell lysate, Mouse kidney tissue, Mouse liver tissueAll lanes: DBT antibody at 4.3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 54 kDaObserved band size: 54 kDa)

Western Blot (WB) (Western BlotPositive WB detected in: 293 whole cell lysate, Mouse kidney tissue, Mouse liver tissueAll lanes: DBT antibody at 4.3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 54 kDaObserved band size: 54 kDa)
Product Categories/Family for anti-DBT antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
53,487 Da
NCBI Official Full Name
lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
NCBI Official Synonym Full Names
dihydrolipoamide branched chain transacylase E2
NCBI Official Symbol
DBT
NCBI Official Synonym Symbols
E2; E2B; BCATE2; BCKADE2; BCKAD-E2; BCOADC-E2
NCBI Protein Information
lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
UniProt Protein Name
Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
UniProt Gene Name
DBT
UniProt Synonym Gene Names
BCATE2; BCOADC-E22 PublicationsManual assertion based on opinion iniRef.14; BCKAD-E2; BCKADE2

NCBI Description

The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component.

Research Articles on DBT

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Product Notes

The DBT dbt (Catalog #AAA7112904) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DBT Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DBT can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 1:500-1:5000. Researchers should empirically determine the suitability of the DBT dbt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DBT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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