Western Blot (WB)
(Western Blot Analysis: Transfected (Lane 1) HEK293/myc-BMAL/empty vector and (Lane 2) HEK293/myc-BMAL1/myc-CLOCK were treated with Nicotinamide. The lysates were resolved by electrophoresis, transferred to PVDF and probed with MBS619476 (1:1,000 dilution). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates BMAL1, acetylated at Lysine 538 (95kD).)
Rabbit anti-Mouse BMAL1 Polyclonal Antibody | anti-BMAL1 antibody
BMAL1, acetylated (Lys538) (aryl hydrocarbon receptor nuclear translocator-like, Aryl hydrocarbon receptor nuclear translocator-like protein 1, Basic-helix-loop-helix-PAS Protein MOP3, bHLHe5, bHLH-PAS Protein JAP3, BMAL1, BMAL1c, Brain and Muscle ARNT-li
Gene Names
ARNTL; TIC; JAP3; MOP3; BMAL1; PASD3; BMAL1c; bHLHe5
Reactivity
Mouse
Applications
Western Blot
Purity
Purified by Immunoaffinity chromatography.
Synonyms
BMAL1; Polyclonal Antibody; acetylated (Lys538) (aryl hydrocarbon receptor nuclear translocator-like; Aryl hydrocarbon receptor nuclear translocator-like protein 1; Basic-helix-loop-helix-PAS Protein MOP3; bHLHe5; bHLH-PAS Protein JAP3; BMAL1c; Brain and Muscle ARNT-li; anti-BMAL1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse BMAL1 acetylated at Lysine 538 at ~95kD. Species sequence homology: rat, opossum, porcine, equine and primate.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-BMAL1 antibody
Western Blot (WB)
Application Notes
WB: 1:1000
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
BMAL1 acetylated at Lysine538 (KLH). Epitope: C-Terminus.
Conjugate
Biotin
Positive Control
Transfected HEK293/myc-BMAL1/empty vector and HEK293/myc-BMAL1/myc-CLOCK treated with Nicotinamide.
Antigen Modification
Acetylated
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot Analysis: Transfected (Lane 1) HEK293/myc-BMAL/empty vector and (Lane 2) HEK293/myc-BMAL1/myc-CLOCK were treated with Nicotinamide. The lysates were resolved by electrophoresis, transferred to PVDF and probed with MBS619476 (1:1,000 dilution). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates BMAL1, acetylated at Lysine 538 (95kD).)
Western Blot (WB)
(Western Blot Analysis: Transfected (Lane 1) HEK293/myc-BMAL/empty vector and (Lane 2) HEK293/myc-BMAL1/myc-CLOCK were treated with Nicotinamide. The lysates were resolved by electrophoresis, transferred to PVDF and probed with MBS619476 (1:1,000 dilution). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates BMAL1, acetylated at Lysine 538 (95kD).)
Product Categories/Family for anti-BMAL1 antibody
References
1. Corsi P., et al. (2007). Nature. 450, 1086-1090.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
626
NCBI Official Full Name
aryl hydrocarbon receptor nuclear translocator-like protein 1 isoform a
NCBI Official Synonym Full Names
aryl hydrocarbon receptor nuclear translocator-like
NCBI Official Symbol
ARNTL
NCBI Official Synonym Symbols
TIC; JAP3; MOP3; BMAL1; PASD3; BMAL1c; bHLHe5
NCBI Protein Information
aryl hydrocarbon receptor nuclear translocator-like protein 1; bHLH-PAS protein JAP3; member of PAS protein 3; member of PAS superfamily 3; brain and muscle ARNT-like 1; PAS domain-containing protein 3; ARNT-like protein 1, brain and muscle; basic-helix-l
UniProt Protein Name
Aryl hydrocarbon receptor nuclear translocator-like protein 1
UniProt Gene Name
ARNTL
UniProt Synonym Gene Names
BHLHE5; BMAL1; MOP3; PASD3; bHLHe5
UniProt Entry Name
BMAL1_HUMAN
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Product Notes
The BMAL1 arntl (Catalog #AAA6465408) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BMAL1, acetylated (Lys538) (aryl hydrocarbon receptor nuclear translocator-like, Aryl hydrocarbon receptor nuclear translocator-like protein 1, Basic-helix-loop-helix-PAS Protein MOP3, bHLHe5, bHLH-PAS Protein JAP3, BMAL1, BMAL1c, Brain and Muscle ARNT-li reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BMAL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the BMAL1 arntl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BMAL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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