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Typical Testing Data

Rat Heparan Sulphate Proteoglycan ELISA Kit | HSPG elisa kit

Rat Heparan Sulphate Proteoglycan ELISA Kit

Gene Names
GPC3; SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2
Reactivity
Rat
Synonyms
Heparan Sulphate Proteoglycan; Rat Heparan Sulphate Proteoglycan ELISA Kit; HSPG/CD362/Fibroglycan/HSPG1/SDC2/SYND2/CD362 antigen/Fibroglycan/fibroglycan/heparan sulfate proteoglycan 1; cell surface-associated/Heparan sulfate proteoglycan core protein/HSPG/HSPG1syndecan proteoglycan 2/SYND2cell surface-associated heparan sulfate proteoglycan 1/syndecan 2/syndecan-2; HSPG elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Sequence Length
580
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
0.313-20ng/ml
Sensitivity
0.188ng/ml
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Testing Data

Typical Standard Curve

Typical Standard Curve
Related Product Information for HSPG elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat HSPG antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat HSPG antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat HSPG amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat HSPG can be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
68,414 Da
NCBI Official Full Name
heparan sulphate proteoglycan
NCBI Official Synonym Full Names
glypican 3
NCBI Official Symbol
GPC3
NCBI Official Synonym Symbols
SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2
NCBI Protein Information
glypican-3
UniProt Protein Name
Glypican-3
UniProt Gene Name
GPC3
UniProt Synonym Gene Names
OCI5
UniProt Entry Name
GPC3_HUMAN

NCBI Description

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]

Uniprot Description

Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.

Research Articles on HSPG

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Product Notes

The Rat HSPG gpc3 (Catalog #AAA762755) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA762755 ELISA Kit recognizes Rat HSPG. It is sometimes possible for the material contained within the vial of "Heparan Sulphate Proteoglycan, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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