Mouse Cytochrome b5 ELISA Kit | CYTB5 elisa kit

Mouse Cytochrome b5 ELISA Kit

Cat. Num. AAA733870

• Gene Names: CYB5A; CYB5; MCB5
• Reactivity: Mouse
• Synonyms: Cytochrome b5; Mouse Cytochrome b5 ELISA Kit; CYTB5 elisa kit

• Reactivity: Mouse

• Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYTB5 elisa kit

For Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma

Intended Uses: This CYB5 ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of CYB5 in the sample, this CYB5 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus CYB5 concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of CYB5 utilizes a multiclonal anti-CYB5 antibody and an CYB5-HRP conjugate. The assay sample and buffer are incubated together with CYB5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CYB5 concentration since CYB5 from samples and CYB5-HRP conjugate compete for the anti-CYB5 antibody binding site. Since the number of sites is limited, as more sites are occupied by CYB5 from the sample, fewer sites are left to bind CYB5-HRP conjugate. Standards of known CYB5 concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of CYB5. The CYB5 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 41281768
• NCBI GeneID: 1528
• NCBI Accession #: NP_683725.1
• NCBI GenBank Nucleotide #: NM_148923.3
• Molecular Weight: 14,169 Da
• NCBI Official Full Name: cytochrome b5 isoform 1
• NCBI Official Synonym Full Names: cytochrome b5 type A (microsomal)
• NCBI Official Symbol: CYB5A
• NCBI Official Synonym Symbols: CYB5; MCB5
• NCBI Protein Information: cytochrome b5; type 1 cyt-b5
• UniProt Protein Name: Cytochrome b5
• Protein Family: Cytochrome
• UniProt Gene Name: CYB5A
• UniProt Synonym Gene Names: CYB5; MCB5
• UniProt Entry Name: CYB5_HUMAN

NCBI Description

The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Uniprot Description

CYB5A: Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. Defects in CYB5A are the cause of methemoglobinemia CYB5A-related (METHB-CYB5A). A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. Belongs to the cytochrome b5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 18q23

Cellular Component: mitochondrial outer membrane; endoplasmic reticulum membrane; membrane; integral to membrane

Molecular Function: cytochrome-c oxidase activity; enzyme binding; metal ion binding; aldo-keto reductase activity; heme binding

Biological Process: vitamin metabolic process; response to cadmium ion; L-ascorbic acid metabolic process; water-soluble vitamin metabolic process

Disease: Methemoglobinemia Type Iv