Typical Testing Data/Standard Curve (for reference only)
Human Regenerating Islet Derived Protein 3 Gamma (REG3g) DIY ELISA Kit | REG3g diy elisa kit
Human Regenerating Islet Derived Protein 3 Gamma (REG3g) ELISA Kit DIY Materials
Gene Names
REG3G; PAP1B; PAPIB; PAP IB; PAP-1B; UNQ429; LPPM429; REG III; REG-III
Reactivity
Human
Synonyms
Regenerating Islet Derived Protein 3 Gamma (REG3g); Human Regenerating Islet Derived Protein 3 Gamma (REG3g) ELISA Kit DIY Materials; REG-III; PAP1B; PAPIB; UNQ429; Pancreatitis-associated protein 1B; Regenerating islet-derived protein III-gamma; REG3g diy elisa kit
Reactivity
Human
Specificity
The Abs in the kit have high sensitivity and excellent specificity for detection of Regenerating Islet Derived Protein 3 Gamma (REG3g). No significant cross-reactivity or interference between Regenerating Islet Derived Protein 3 Gamma (REG3g) and analogues was observed.
Sequence Length
175
Application Notes
Main materials for "Do It Yourself ELISA Kit"
Assay Type
Double-antibody Sandwich ELISA for Antigen Detection
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
0.064ng/mL
Reagent Contents
Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage
1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required
ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
Preparation and Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Product Categories/Family for REG3g diy elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,409 Da
NCBI Official Full Name
regenerating islet-derived protein 3-gamma isoform 1 preproprotein
NCBI Official Synonym Full Names
regenerating family member 3 gamma
NCBI Official Symbol
REG3G
NCBI Official Synonym Symbols
PAP1B; PAPIB; PAP IB; PAP-1B; UNQ429; LPPM429; REG III; REG-III
NCBI Protein Information
regenerating islet-derived protein 3-gamma
UniProt Protein Name
Regenerating islet-derived protein 3-gamma
Protein Family
UniProt Gene Name
REG3G
UniProt Synonym Gene Names
PAP1B; REG-3-gamma; PAP-1B; PAP IB; REG III; Reg III-gamma
NCBI Description
This gene encodes a member of the regenerating islet-derived genes (REG)3 protein family. These proteins are secreted, C-type lectins with a carbohydrate recognition domain and N-terminal signal peptide. The protein encoded by this gene is an antimicrobial lectin with activity against Gram-positive bacteria. Alternative splicing results in multiple transcript variants encoding multiple isoforms. [provided by RefSeq, Nov 2014]
Uniprot Description
REG3G: Might be a stress protein involved in the control of bacterial proliferation.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 2p12
Biological Process: defense response to Gram-positive bacterium; MyD88-dependent toll-like receptor signaling pathway; negative regulation of keratinocyte differentiation
Research Articles on REG3g
Similar Products
Product Notes
The Human REG3g reg3g (Catalog #AAA2089361) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2089361 DIY ELISA Kit recognizes Human REG3g. Main materials for "Do It Yourself ELISA Kit". Researchers should empirically determine the suitability of the REG3g reg3g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Regenerating Islet Derived Protein 3 Gamma (REG3g), DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
