Canine Cytochrome b5 ELISA Kit | CYTB5 elisa kit

Canine Cytochrome b5 ELISA Kit

Cat. Num. AAA744655

• Gene Names: CYB5A; CYB5; MCB5
• Reactivity: Canine
• Synonyms: Cytochrome b5; Canine Cytochrome b5 ELISA Kit; CYTB5 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This CYTB5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine CYTB5. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This CYTB5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine CYTB5. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYTB5 elisa kit

Principle of the Assay: CYTB5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CYTB5 antibody and an CYTB5-HRP conjugate. The assay sample and buffer are incubated together with CYTB5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CYTB5 concentration since CYTB5 from samples and CYTB5-HRP conjugate compete for the anti-CYTB5 antibody binding site. Since the number of sites is limited, as more sites are occupied by CYTB5 from the sample, fewer sites are left to bind CYTB5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYTB5 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 41281768
• NCBI GeneID: 1528
• NCBI Accession #: NP_683725.1
• NCBI GenBank Nucleotide #: NM_148923.3
• Molecular Weight: 14,169 Da
• NCBI Official Full Name: cytochrome b5 isoform 1
• NCBI Official Synonym Full Names: cytochrome b5 type A (microsomal)
• NCBI Official Symbol: CYB5A
• NCBI Official Synonym Symbols: CYB5; MCB5
• NCBI Protein Information: cytochrome b5; type 1 cyt-b5
• UniProt Protein Name: Cytochrome b5
• Protein Family: Cytochrome
• UniProt Gene Name: CYB5A
• UniProt Synonym Gene Names: CYB5; MCB5
• UniProt Entry Name: CYB5_HUMAN

NCBI Description

The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Uniprot Description

CYB5A: Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. Defects in CYB5A are the cause of methemoglobinemia CYB5A-related (METHB-CYB5A). A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. Belongs to the cytochrome b5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 18q23

Cellular Component: endoplasmic reticulum membrane; mitochondrial outer membrane; membrane; integral to membrane

Molecular Function: cytochrome-c oxidase activity; enzyme binding; aldo-keto reductase activity; metal ion binding; heme binding

Biological Process: vitamin metabolic process; response to cadmium ion; L-ascorbic acid metabolic process; water-soluble vitamin metabolic process

Disease: Methemoglobinemia Type Iv