Rabbit CHRM3 / M3 Polyclonal Antibody | anti-CHRM3 antibody

Rabbit Polyclonal to Human CHRM3 / M3

Cat. Num. AAA240007

• Gene Names: CHRM3; HM3; EGBRS
• Reactivity: Gibbon, Chimpanzee, Gorilla, Human, Monkey, Orangutan; Predicted Reactivity: Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Sheep (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: CHRM3 / M3; Polyclonal Antibody; Rabbit Polyclonal to Human CHRM3 / M3; Anti-CHRM3 / M3 Antibody (Cytoplasmic Domain) IHC-plus; CHRM3; M3; Muscarinic receptor M3; Muscarinic M3 receptor; EGBRS; HM3; M3 muscarinic receptor; Human CHRM3; anti-CHRM3 antibody

• Host: Rabbit
• Reactivity: Gibbon, Chimpanzee, Gorilla, Human, Monkey, Orangutan; Predicted Reactivity: Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Sheep (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human CHRM3 / M3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 590

• Applicable Applications for anti-CHRM3 antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA, IHC-P (10 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic 20 amino acid peptide from 3rd cytoplasmic domain of human CHRM3 / M3. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: CHRM3 / M3 antibody was raised against synthetic 20 amino acid peptide from 3rd cytoplasmic domain of human CHRM3 / M3. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%); Mouse, Rat, Sheep, Bovine, Hamster, Elephant, Horse, Rabbit, Pig (95%); Dog, Opossum (90%); Lizard (85%); Panda, Guinea pig (80%).
• Antigen Modification: Cytoplasmic Domain
• Preparation and Storage: Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC)

(Anti-CHRM3 / M3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-CHRM3 antibody

Muscarinic Acetylcholine Receptor 3 (CHRM3) is an Acetylcholine Receptor that controls smooth muscle contraction by mediating phosphoinositide hydrolysis and Ca2+ mobilization. Activation of CHRM3 causes secretion in glandular tissues. Mouse studies suggest that the M3 receptor may play a role in appetite stimulation and food intake.

Product Categories/Family for anti-CHRM3 antibody

Family: GPCR; Subfamily: Acetylcholine

NCBI and Uniprot Product Information

• NCBI GI #: 4502819
• NCBI GeneID: 1131
• NCBI Accession #: NP_000731.1
• NCBI GenBank Nucleotide #: NM_000740.2
• UniProt Accession #: P20309; Q0VAJ8; Q4QRI3; Q5VXY2; Q9HB60
• Molecular Weight: 66,128 Da
• NCBI Official Full Name: muscarinic acetylcholine receptor M3
• NCBI Official Synonym Full Names: cholinergic receptor, muscarinic 3
• NCBI Official Symbol: CHRM3
• NCBI Official Synonym Symbols: HM3; EGBRS
• NCBI Protein Information: muscarinic acetylcholine receptor M3; m3 muscarinic receptor; acetylcholine receptor, muscarinic 3
• UniProt Protein Name: Muscarinic acetylcholine receptor M3
• Protein Family: Muscarinic acetylcholine receptor
• UniProt Gene Name: CHRM3
• UniProt Entry Name: ACM3_HUMAN

NCBI Description

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. [provided by RefSeq, Jul 2008]

Uniprot Description

mAChR M3: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. Defects in CHRM3 are the cause of Eagle-Barrett syndrome (EGBRS). EGBRS is a syndrome characterized by thin abdominal musculature with overlying lax skin, cryptorchism, megacystis with disorganized detrusor muscle, and urinary tract abnormalities. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.

Protein type: Receptor, GPCR; Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: asymmetric synapse; postsynaptic membrane; integral to plasma membrane; basolateral plasma membrane; dendrite; plasma membrane; nerve terminal; cell junction

Molecular Function: protein binding; receptor activity; drug binding; acetylcholine binding; phosphoinositide phospholipase C activity; G-protein coupled acetylcholine receptor activity

Biological Process: cell proliferation; G-protein coupled receptor protein signaling pathway; nervous system development; smooth muscle contraction; acetylcholine receptor signaling, muscarinic pathway; regulation of vascular smooth muscle contraction; energy reserve metabolic process; protein modification process; positive regulation of smooth muscle contraction; signal transduction; regulation of insulin secretion; saliva secretion

Disease: Abdominal Muscles, Absence Of, With Urinary Tract Abnormality And Cryptorchidism