Mouse anti-Human CRX Monoclonal Antibody | anti-CRX antibody

CRX (Cone-rod Homeobox Protein, CORD2) (AP)

Cat. Num. AAA6130759

• Gene Names: CRX; CRD; LCA7; OTX3; CORD2
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CRX; Monoclonal Antibody; CRX (Cone-rod Homeobox Protein; CORD2) (AP); anti-CRX antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: F6-C2
• Specificity: Recognizes human CRX.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CRX antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-300 from human CRX (AAH16664) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MMAYMNPGPHYSVNALALSGPSVDLMHQAVPYPSAPRKQRRERTTFTRSQLEELEALFAKTQYPDVYAREEVALKINLPESRVQVWFKNRRAKCRQQRQQQKQQQQPPGGQAKARPAKRKAGTSPRPSTDVCPDPLGISDSYSPPLPGPSGSPTTAVATVSIWSPASESPLPEAQRAGLVASGPSLTSAPYAMTYAPASAFCSSPSAYGSPSSYFSGLDPYLSPMVPQLGGPALSPLSGPSVGPSLAQSPTSLSGQSYGAYSPVDSLEFKDPTGTWKFTYNPMDPLDYKDQSAWKFQIL
• Conjugate: AP
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (42.5kD).)

Western Blot (WB)

(Western Blot analysis of CRX expression in transfected 293T cell line by CRX monoclonal antibody. Lane 1: CRX transfected lysate (32.261kD). Lane 2: Non-transfected lysate.)

Testing Data

(Detection limit for recombinant GST tagged CRX is ~1ng/ml as a capture antibody.)

Western Blot (WB)

(Western blot analysis of CRX over-expressed 293 cell line, cotransfected with CRX Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CRX monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Related Product Information for anti-CRX antibody

Binds and transactivates the sequence 5'-TAATC[CA]-3' which is found upstream of several photoreceptor-specific genes, including the opsin genes. Acts synergistically with other transcription factors, e.g. NRL and RX, to regulate photoreceptor cell-specific gene transcription. Essential for the maintenance of mammalian photoreceptors.

Product Categories/Family for anti-CRX antibody

Antibodies; Transcription Factors; Homeobox (HOX)

References

1. Effects of Extracellular Matrix and Neighboring Cells on Induction of Human Embryonic Stem Cells into Retinal or Retinal Pigment Epithelial Progenitors. Gonga J, Sagiva O, Caia H, Tsanga SH, Del Priore LV.Exp Eye Res. 2008 Jun;86(6):957-65. Epub 2008 Mar 28.

NCBI and Uniprot Product Information

• NCBI GI #: 16741737
• NCBI GeneID: 1406
• Molecular Weight: 32,261 Da
• NCBI Official Full Name: Homo sapiens cone-rod homeobox, mRNA
• NCBI Official Synonym Full Names: cone-rod homeobox
• NCBI Official Symbol: CRX
• NCBI Official Synonym Symbols: CRD; LCA7; OTX3; CORD2
• NCBI Protein Information: cone-rod homeobox protein
• Protein Family: Cone-rod homeobox protein

NCBI Description

The protein encoded by this gene is a photoreceptor-specific transcription factor which plays a role in the differentiation of photoreceptor cells. This homeodomain protein is necessary for the maintenance of normal cone and rod function. Mutations in this gene are associated with photoreceptor degeneration, Leber congenital amaurosis type III and the autosomal dominant cone-rod dystrophy 2. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]