Rat NADH-cytochrome b5 reductase 3 ELISA Kit | Cyb5r3 elisa kit

Rat NADH-cytochrome b5 reductase 3 ELISA Kit

Cat. Num. AAA763720

• Gene Names: Cyb5r3; Dia1; Nadhcb5; RNNADHCB5
• Reactivity: Rat
• Synonyms: NADH-cytochrome b5 reductase 3; Rat NADH-cytochrome b5 reductase 3 ELISA Kit; Cyb5r3; Cyb5r3 elisa kit

• Reactivity: Rat
• Sequence Length: 301

• Assay Type: Sandwich
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Cyb5r3 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 20302049
• NCBI GeneID: 25035
• NCBI Accession #: NP_620232.1
• NCBI GenBank Nucleotide #: NM_138877.1
• Molecular Weight: 31,592 Da
• NCBI Official Full Name: NADH-cytochrome b5 reductase 3
• NCBI Official Synonym Full Names: cytochrome b5 reductase 3
• NCBI Official Symbol: Cyb5r3
• NCBI Official Synonym Symbols: Dia1; Nadhcb5; RNNADHCB5
• NCBI Protein Information: NADH-cytochrome b5 reductase 3
• UniProt Protein Name: NADH-cytochrome b5 reductase 3
• Protein Family: NADH-cytochrome b5 reductase
• UniProt Gene Name: Cyb5r3
• UniProt Synonym Gene Names: Dia1; B5R; Cytochrome b5 reductase
• UniProt Entry Name: NB5R3_RAT

NCBI Description

catalyzes the conversion of NADH, H+, and 2 ferricytochrome b(5) to NAD+ and 2 ferrocytochrome b(5) [RGD, Feb 2006]

Uniprot Description

CYB5R3: Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Defects in CYB5R3 are the cause of methemoglobinemia CYB5R3-related (METHB-CYB5R3). A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. There are two types of methemoglobinemia CYB5R3-related. In type 1, the defect affects the soluble form of the enzyme, is restricted to red blood cells, and causes well- tolerated methemoglobinemia. In type 2, the defect affects both the soluble and microsomal forms of the enzyme and is thus generalized, affecting red cells, leukocytes and all body tissues. Type 2 methemoglobinemia is associated with mental deficiency and other neurologic symptoms. Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: EC 1.6.2.2; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Oxidoreductase; Mitochondrial

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; lipid particle; membrane; mitochondrial inner membrane; mitochondrial outer membrane; mitochondrion

Molecular Function: ADP binding; AMP binding; cytochrome-b5 reductase activity; FAD binding; NAD binding

Biological Process: cholesterol biosynthetic process