Human NADH-cytochrome b5 reductase 2 ELISA Kit | CYB5R2 elisa kit

Human NADH-cytochrome b5 reductase 2 ELISA Kit

Cat. Num. AAA763403

• Gene Names: CYB5R2; B5R.2
• Reactivity: Human
• Synonyms: NADH-cytochrome b5 reductase 2; Human NADH-cytochrome b5 reductase 2 ELISA Kit; CYB5R2/NADH-cytochrome b5 reductase 2/b52; CYB5R2 elisa kit

• Reactivity: Human
• Sequence Length: 276

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CYB5R2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human CYB5R2antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human CYB5R2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CYB5R2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman CYB5R2can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 47778923
• NCBI GeneID: 51700
• NCBI Accession #: NP_057313.2
• NCBI GenBank Nucleotide #: NM_016229.4
• Molecular Weight: 27,043 Da
• NCBI Official Full Name: NADH-cytochrome b5 reductase 2 isoform 1
• NCBI Official Synonym Full Names: cytochrome b5 reductase 2
• NCBI Official Symbol: CYB5R2
• NCBI Official Synonym Symbols: B5R.2
• NCBI Protein Information: NADH-cytochrome b5 reductase 2
• UniProt Protein Name: NADH-cytochrome b5 reductase 2
• Protein Family: NADH-cytochrome b5 reductase
• UniProt Gene Name: CYB5R2
• UniProt Synonym Gene Names: b5R.2
• UniProt Entry Name: NB5R2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the flavoprotein pyridine nucleotide cytochrome reductase family of proteins. Cytochrome b-type NAD(P)H oxidoreductases are implicated in many processes including cholesterol biosynthesis, fatty acid desaturation and elongation, and respiratory burst in neutrophils and macrophages. Cytochrome b5 reductases have soluble and membrane-bound forms that are the product of alternative splicing. In animal cells, the membrane-bound form binds to the endoplasmic reticulum, where it is a member of a fatty acid desaturation complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

Uniprot Description

CYB5R2: NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4- disulfophenyl]-2H tetrazolium monosodium salt (WST-1). Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.6.2.2; Oxidoreductase

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: membrane; mitochondrion; nucleus

Molecular Function: cytochrome-b5 reductase activity; protein binding

Biological Process: sterol biosynthetic process