Rabbit Gle1 Polyclonal Antibody | anti-Gle1 antibody

Gle1 Antibody

Cat. Num. AAA9404385

• Gene Names: GLE1; LCCS; GLE1L; LCCS1; hGLE1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: Gle1; Polyclonal Antibody; Gle1 Antibody; RNA export mediator; anti-Gle1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 698

• Applicable Applications for anti-Gle1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 15 amino acid peptide near the carboxy terminus of human Gle1.
• Target Name: Gle1
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Gle1 in mouse brain tissue lysate with Gle1 antibody at (A) 1 and (B) 2 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of Gle1 in mouse brain tissue with Gle1 antibody at 2.5 ug/mL.)

Related Product Information for anti-Gle1 antibody

The proper expression of gene products in eukaryotic cells relies on efficient transport of mRNA molecules out of the nucleus. Gle1 is an essential mRNA export factor in both human and yeast cells. It associates with the nuclear pore complex (NPC) through hCG1 and NUP155 in mammalian cells and in conjunction with inositol hexakisphosphate (IP6), stimulates Dbp5, a member of the DEAD-box helicase family, triggering mRNP remodeling and facilitating RNA export from the nucleus. Recent evidence suggests that mutations in Gle1 causing defects in mRNA export can result in human disease. At least three isoforms of Gle1 are known to exist.

Product Categories/Family for anti-Gle1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 51317384
• NCBI GeneID: 2733
• NCBI Accession #: NP_001003722
• NCBI GenBank Nucleotide #: NM_001003722.1
• Molecular Weight: 75,407 Da
• NCBI Official Full Name: nucleoporin GLE1 isoform 1
• NCBI Official Synonym Full Names: GLE1 RNA export mediator
• NCBI Official Symbol: GLE1
• NCBI Official Synonym Symbols: LCCS; GLE1L; LCCS1; hGLE1
• NCBI Protein Information: nucleoporin GLE1
• UniProt Protein Name: Nucleoporin GLE1
• Protein Family: Protein
• UniProt Gene Name: GLE1
• UniProt Synonym Gene Names: GLE1L; hGLE1
• UniProt Entry Name: GLE1_HUMAN

NCBI Description

This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GLE1: Required for the export of mRNAs containing poly(A) tails from the nucleus into the cytoplasm. May be involved in the terminal step of the mRNA transport through the nuclear pore complex (NPC). Defects in GLE1 are the cause of lethal congenital contracture syndrome type 1 (LCCS1); also known as multiple contracture syndrome type Finnish. LCCS is an autosomal recessive disorder characterized by early fetal hydrops and akinesia, micrognatia, pulmonary hypoplasia, pterygia, multiple joint contractures, specific neuropathology with degeneration of anterior horn neurons and extreme skeletal muscle atrophy. LCCS1 leads to prenatal death. Defects in GLE1 are the cause of lethal arthrogryposis with anterior horn cell disease (LAAHD). LAAHD is characterized by fetal akinesia, arthrogryposis and motor neuron loss. LAADH fetus often survive delivery, but die early as a result of respiratory failure. Neuropathological findings resemble those of LCCS1, but are less severe. Belongs to the GLE1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear export; Karyopherin

Chromosomal Location of Human Ortholog: 9q34.11

Cellular Component: extracellular space; membrane; cytoplasm; plasma membrane; nuclear pore

Molecular Function: identical protein binding; protein binding

Biological Process: protein transport; mRNA export from nucleus; poly(A)+ mRNA export from nucleus

Disease: Lethal Arthrogryposis With Anterior Horn Cell Disease; Lethal Congenital Contracture Syndrome 1