IP-10 recombinant protein

Human IP-10 Recombinant

Cat. Num. AAA553046

• Purity: >97%, as determined by SDS-PAGE and HPLC
• Synonyms: IP-10; Human IP-10 Recombinant; ip10; ip-10; cxcl10; IP-10 recombinant protein

• Host: E Coli
• Purity/Purification: >97%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant IP-10/CXCL10 was lyophilized from 0.2 um filtered PBS solution, pH 7.0.
• Sequence: MNQTAILICC LIFLTLSGIQ GVPLSRTVRC TCISISNQPV NPRSLEKLEI IPASQFCPRV EIIATMKKKG EKRCLNPESK AIKNLLKAVS KERSKRSP
• Sequence Length: 98

• Domain: IL8
• Host Note: Optimized DNA sequence encoding Human IP-10 mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native human IP-10/CXCL10, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 9 kDa.; ; Recombinant IP-10 is a disulfide-linked homodimeric protein consisting of 78 amino acid residue subunits, and migrates as an approximately 9 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for IP-10 recombinant protein

IP10 shows homology to PF4 (platelet factor-4) and belongs to the family of chemotactic cytokines known as Chemokines. The receptor for IP-10 is CXCR3. IP-10 has been shown to bind to the virus-encoded viroceptor M3. The expression of IP-10 from a variety of cells, including monocytes, endothelial cells, keratinocytes, and fibroblasts, is induced by IFN-gamma and TNF-alpha. Human neutrophils produce IP-10 in response to IFN- gamma in combination with either TNF-alpha or bacterial lipopolysaccharides. IP-10 probably also plays a role in regulation of the growth of immature hematopoietic progenitor cells. It has been shown to suppress in vitro colony formation of highly enriched cells expressing the cell surface marker CD34 in the presence of SCF, GM-CSF, or SCF and EPO, but not in their absence with the exception of SCF.

Product Categories/Family for IP-10 recombinant protein

Cytokines

References

• role of t cell chemoattractants cxcl10/IP10 and cxcl9/mig in high risk corneal allograft transplantation .Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 1977.
• role of cxcl10/IP10 production in high risk corneal allograft transplantation .Invest. Ophthalmol. Vis. Sci., Apr 2008; 49: 3721.
• short-lived, mhcii positive plasmablasts, but not long-lived plasma cells from different aged nzb/w mice migrate to IP10 and sdf1 alpha .Ann Rheum Dis, Jun 2007; 66: 307.
• the ligands of cxc chemokine receptor 3, i-tac, mig, and IP10, are natural antagonists for ccr3 .J. Biol. Chem., Jan 2001; 276: 2986 - 2991.