Medium 199 w/ Earle's w/o L-Glutamine Culture Media
Medium 199 w/ Earle's w/o L-Glutamine (Powder, TC 199)
Purity
Cell Culture Grade
Synonyms
Medium 199 w/ Earle's w/o L-Glutamine; Medium 199 w/ Earle's w/o L-Glutamine (Powder; TC 199); Medium 199 w/ Earle's w/o L-Glutamine culture media
Purity/Purification
Cell Culture Grade
Form/Format
Light pink, homogenous, free flowing powder
Endotoxin Level
<= 1EU/ml
pH
4.9
Solubility
Yellow, clear, complete
Directions per Liter
Dissolve 9.4g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add 0.35g sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-liable compounds that can be damaged with autoclaving.
Components shown as mg/liter:
Amino Acids
Amino Acids
L-Alanine: 25
L-Arginine•HCl: 70
L-Aspartic Acid: 30
L-Cysteine•HCl•H2O: 0.11
L-Cystine•2HCl: 26
L-Glutamic Acid: 75
Glycine: 50
L-Histidine•HCl•H2O: 21.88
Hydroxy-L-Proline: 10
L-Isoleucine: 20
L-Leucine: 60
L-Lysine•HCl: 70
L-Methionine: 15
L-Phenylalanine: 25
L-Proline: 40
L-Serine: 25
L-Threonine: 30
L-Tryptophan: 10
L-Tyrosine•2Na•2H2O: 57.66
L-Valine 25
L-Arginine•HCl: 70
L-Aspartic Acid: 30
L-Cysteine•HCl•H2O: 0.11
L-Cystine•2HCl: 26
L-Glutamic Acid: 75
Glycine: 50
L-Histidine•HCl•H2O: 21.88
Hydroxy-L-Proline: 10
L-Isoleucine: 20
L-Leucine: 60
L-Lysine•HCl: 70
L-Methionine: 15
L-Phenylalanine: 25
L-Proline: 40
L-Serine: 25
L-Threonine: 30
L-Tryptophan: 10
L-Tyrosine•2Na•2H2O: 57.66
L-Valine 25
Inorganic Salts
Calcium Chloride: 200
Ferric Nitrate•9H2O: 0.72
Magnesium Sulfate: 97.7
Potassium Chloride: 400
Sodium Acetate: 50
Sodium Chloride: 6800
Sodium Phosphate Mono: 140
Ferric Nitrate•9H2O: 0.72
Magnesium Sulfate: 97.7
Potassium Chloride: 400
Sodium Acetate: 50
Sodium Chloride: 6800
Sodium Phosphate Mono: 140
Vitamins
Adenine Sulfate: 10
p-Aminobenzoic Acid: 0.05
L-Ascorbic Acid: 0.05
D-Biotin: 0.01
Choline Chloride: 0.5
Calciferol: 0.1
Folic Acid: 0.01
i-Inositol: 0.05
Menadione, NaBisulfate•3H2O: 0.019
Niacinamide: 0.025
D-Pantothenic Acid•Ca: 0.01
Pyridoxine•HCl: 0.05
Riboflavin: 0.01
Thiamine•HCl: 0.01
DL-a-Tocopherol•PO4•2Na: 0.01
Vitamin A 9Retinol) Acetate: 0.14
p-Aminobenzoic Acid: 0.05
L-Ascorbic Acid: 0.05
D-Biotin: 0.01
Choline Chloride: 0.5
Calciferol: 0.1
Folic Acid: 0.01
i-Inositol: 0.05
Menadione, NaBisulfate•3H2O: 0.019
Niacinamide: 0.025
D-Pantothenic Acid•Ca: 0.01
Pyridoxine•HCl: 0.05
Riboflavin: 0.01
Thiamine•HCl: 0.01
DL-a-Tocopherol•PO4•2Na: 0.01
Vitamin A 9Retinol) Acetate: 0.14
Other
5'-Adenylic acid: 0.2
Adenosine Triphosphate•2Na: 1
Cholesterol: 0.2
2'-Deoxy-D-ribose: 0.5
Thymine: 0.3
D-Glucose: 1000
Glutathione: 0.05
Guanine•HCl: 0.3
Hypoxanthine (Na): 0.35
Phenol Red, Sodium: 10
Tween 80: 5
D-Ribose: 0.5
Uracil: 0.3
Xanthine•Na: 0.34
Adenosine Triphosphate•2Na: 1
Cholesterol: 0.2
2'-Deoxy-D-ribose: 0.5
Thymine: 0.3
D-Glucose: 1000
Glutathione: 0.05
Guanine•HCl: 0.3
Hypoxanthine (Na): 0.35
Phenol Red, Sodium: 10
Tween 80: 5
D-Ribose: 0.5
Uracil: 0.3
Xanthine•Na: 0.34
Total
9.4g/liter
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Related Product Information for Medium 199 w/ Earle's w/o L-Glutamine culture media
In 1950, Morgan, Morton and Parker described one of the first totally defined media that did not depend largely on animal products or extracts as nutritive sources. This medium supported the growth of primary chick embryo heart cells. It has since become known as Medium 199. Its current use is with the addition of serum in virus and vaccine production, and in the culture of many non-transformed cells. It is also used in combination with less complex media. Medium 199 modified with Earle's Balanced Salts is designed for use with cells maintained in ambient (non-CO2) atmospheric conditions. M2852 differs from the original formulation by containing all L-amino acids. The original formulation contained 10 DL-amino acids, which are less biologically active on a per gram basis than the corresponding L-amino acids.
The search for a synthetic medium to replace serum for maintaining cells in vitro began in the late nineteenth century and continues to this day. Ringer, Locks and Tyrode substituted physiological salt solutions augmented with glucose for serum and thereby laid the foundation for the development of defined media. As biochemical and analytical techniques have improved, more of the components in serum such as vitamins, hormones, and amino acids have been identified and incorporated into physiological salt solutions, reducing, and in some cases eliminating the concentrations of animal sera required as a medium supplement. Although there have been many modifications to the original formulas in efforts to produce fully defined media, salt solutions still play an important role in tissue culture. A salt solution's basic function, to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions, is as valuable today as when it was first developed a century ago. Earle's Balanced Salts selection is strongly influenced by 1. type of cell, 2. type of culture monolayer, suspension, clonal, and 3. degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.
The search for a synthetic medium to replace serum for maintaining cells in vitro began in the late nineteenth century and continues to this day. Ringer, Locks and Tyrode substituted physiological salt solutions augmented with glucose for serum and thereby laid the foundation for the development of defined media. As biochemical and analytical techniques have improved, more of the components in serum such as vitamins, hormones, and amino acids have been identified and incorporated into physiological salt solutions, reducing, and in some cases eliminating the concentrations of animal sera required as a medium supplement. Although there have been many modifications to the original formulas in efforts to produce fully defined media, salt solutions still play an important role in tissue culture. A salt solution's basic function, to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions, is as valuable today as when it was first developed a century ago. Earle's Balanced Salts selection is strongly influenced by 1. type of cell, 2. type of culture monolayer, suspension, clonal, and 3. degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.
Product Categories/Family for Medium 199 w/ Earle's w/o L-Glutamine culture media
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Product Notes
The Medium 199 w/ Earle's w/o L-Glutamine (Catalog #AAA653449) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Medium 199 w/ Earle's w/o L-Glutamine, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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