BPIFB1 / LPLUNC1 recombinant protein

Recombinant Human BPIFB1/LPLUNC1 Protein (His Tag)

Cat. Num. AAA2545342

• Gene Names: BPIFB1; LPLUNC1; C20orf114
• Purity: > 95 % as determined by reducing SDS-PAGE.
• Synonyms: BPIFB1 / LPLUNC1; Recombinant Human BPIFB1/LPLUNC1 Protein (His Tag); BPIFB1; C20orf114; LPLUNC1; BPIFB1 / LPLUNC1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: > 95 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.
• Sequence: Met 1-Gln484

• Application Notes: The recombinant human BPIFB1 consists of 474 amino acids and predicts a molecular mass of 51.7 KDa. It migrates as an approximately 53 KDa band in SDS-PAGE under reducing conditions.
• Species: Human
• Tag: C-His
• Endotoxin: < 1.0 EU per ug as determined by the LAL method.
• Reconstitution: It is recommended that sterile water(70ul) be added to the vial to prepare a stock solution of 0.15mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; ; Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

SDS-PAGE

Related Product Information for BPIFB1 / LPLUNC1 recombinant protein

Background: BPIFB1, also known as LPLUNC1, belongs to the BPI/LBP/Plunc superfamily, plunc family. BPIFB1 may be involved in the innate immune response to bacterial exposure in the mouth, nasal cavities, and lungs. BPIFB1 is expressed in the upper respiratory tract and oral cavity, which may function in host defence. The expression of BPIF proteins is associated with CF lung disease in humans and mice. It is unclear if this elevation of protein production, which results from phenotypic alteration of the cells within the diseased epithelium, plays a role in the pathogenesis of the disease. BPIFB1 is an abundant, secreted product of goblet cells and minor mucosal glands of the respiratory tract and oral cavity and suggest that the protein functions in the complex milieu that protects the mucosal surfaces in these locations.

Description: A DNA sequence encoding the human BPIFB1 (AAH08429.1) (Met1-Gln484) was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 14250058
• NCBI GeneID: 92747
• Molecular Weight: Mol_Mass: 51.7 kDa; AP_Mol_Mass: 53 kDa
• NCBI Official Full Name: Chromosome 20 open reading frame 114
• NCBI Official Synonym Full Names: BPI fold containing family B member 1
• NCBI Official Symbol: BPIFB1
• NCBI Official Synonym Symbols: LPLUNC1; C20orf114
• NCBI Protein Information: BPI fold-containing family B member 1
• UniProt Protein Name: BPI fold-containing family B member 1
• UniProt Gene Name: BPIFB1
• UniProt Synonym Gene Names: C20orf114; LPLUNC1; VEMSGP

NCBI Description

The protein encoded by this gene may be involved in the innate immune response to bacterial exposure in the mouth, nasal cavities, and lungs. The encoded protein is secreted and is a member of the BPI/LBP/PLUNC protein superfamily. This gene is found with other members of the superfamily in a cluster on chromosome 20. [provided by RefSeq, Jul 2008]

Uniprot Description

LPLUNC1: May play a role in innate immunity in mouth, nose and lungs. Belongs to the BPI/LBP/Plunc superfamily. Plunc family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q11.21

Cellular Component: extracellular region

Biological Process: innate immune response in mucosa; negative regulation of toll-like receptor 4 signaling pathway