Endothelial Basal Medium MCDB 131 w/ D-Valine, low Phenol Red Culture Media
Endothelial Basal Medium MCDB 131 w/ D-Valine, low Phenol Red (Powder)
Purity
Cell Culture Grade
Synonyms
Endothelial Basal Medium MCDB 131 w/ D-Valine; low Phenol Red; low Phenol Red (Powder); low Phenol Red culture media
Purity/Purification
Cell Culture Grade
Form/Format
Pale orange, homogenous, free-flowing powder
Solubility
Pink, clear, complete
pH
7.4
Endotoxin
< 1 EU/ml
Directions per Liter
Dissolve 11.52g in 800-900ml of ddH2O, stirring gently until completely solubilized. Add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Components shown as mg/L
Inorganic Salts
Ammonium Metavanadate 0.0006
Ammonium Molybdate•4H2O 0.0037
Calcium Chloride•2H2O 235
Cupric Sulfate•5H2O 0.0012
Ferrous Sulfate•7H2O 0.283
Magnesium Sulfate•7H2O 2464
Manganese Sulfate•H20 0.0002
Nickel Chloride•6H2O 0.000071
Potassium Chloride 298
Sodium Chloride 6430
Sodium Meta Silicate•9H2O 2.8
Sodium Phosphate Dibasic•7H2O 134
Zinc Sulfate•H2O 0.0003
Ammonium Metavanadate 0.0006
Ammonium Molybdate•4H2O 0.0037
Calcium Chloride•2H2O 235
Cupric Sulfate•5H2O 0.0012
Ferrous Sulfate•7H2O 0.283
Magnesium Sulfate•7H2O 2464
Manganese Sulfate•H20 0.0002
Nickel Chloride•6H2O 0.000071
Potassium Chloride 298
Sodium Chloride 6430
Sodium Meta Silicate•9H2O 2.8
Sodium Phosphate Dibasic•7H2O 134
Zinc Sulfate•H2O 0.0003
Amino Acids
L-Alanine 2.7
L-Arginine Hydrochloride 63.2
L-Asparagine•H2O 15
L-Aspartic Acid 13.3
L-Cysteine•2HCl.•H2O 35
L-Glutamic Acid 4.4
Glycine 2.3
L-Histidine Hydrochloride•H2O 42
L-Isoleucine 66
L-Leucine 131
L-Lysine Hydrochloride 182
L-Methionine 15
L-Phenylalanine 33
L-Proline 11.5
L-Serine 32
L-Threonine 12
L-Tryptophan 4.1
L-Tyrosine 18.1
D-Valine 117
Vitamins
Biotin 0.0073
Choline Chloride 14
Vitamin B12 0.0136
Folinic Acid Calcium 0.6
i-Inositol 7.2
Niacinamide 6.1
D-Calcium Pantothenate 12
Pyridoxine Hydrochloride 2.1
Riboflavin 0.0038
Thiamine Hydrochloride 3.4
Total: 11.52g/L
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Related Product Information for Endothelial Basal Medium MCDB 131 w/ D-Valine, low Phenol Red culture media
MCDB 131 was developed by Knedler and Ham as a reduced serum-supplemented medium for the culture of human microvascular endothelial cells. This formulation has been reported to support clonal growth of human omental microvascular cells when supplemented with 0.7% dialyzed FBS, 10ng/mL EGF, and 1ug/mL hydrocortisone.
Product Categories/Family for Endothelial Basal Medium MCDB 131 w/ D-Valine, low Phenol Red culture media
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Product Notes
The Endothelial Basal Medium MCDB 131 w/ D-Valine, low Phenol Red (Catalog #AAA653192) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Endothelial Basal Medium MCDB 131 w/ D-Valine, low Phenol Red, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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