Cathepsin S / CTSS Recombinant Protein | CTSS recombinant protein

Recombinant Human Cathepsin S / CTSS Protein (His tag)

Cat. Num. AAA2546225

• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: Cathepsin S / CTSS; Recombinant Human Cathepsin S / CTSS Protein (His tag); MGC3886; CTSS; CTSS recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 331

• Application Notes: The secreted recombinant human CTSS consists of 326 amino acids with the predicted molecular mass of 37 kDa as estimated by SDS-PAGE under reducing conditions.
• Predicted N Terminal: Gln 17
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CTSS recombinant protein

Background: Cathepsin S (CTSS), one of the lysosomal proteinases, has many important physiological functions in the nervous system, especially in process of extracellular matrix degradation and endocellular antigen presentation. CTSS is synthesized as inactive precursor of 331 amino acids consisting of a 15-aa signal peptide, a propeptide of 99 aa, and a mature polypeptide of 217 aa. It is activated in the lysosomes by a proteolytic cleavage of the propeptide. Cathepsin S is expressed in the lysosome of antigen presenting cells, primarily dendritic cells, B-cells and macrophages. Compared with other lysosomal cysteine proteases, cathepsin S has displayed some unique characteristics. Cathepsin S is most well known for its critical function in the proteolytic digestion of the invariant chain chaperone molecules, thus controlling antigen presentation to CD4+ T-cells by major histocompatibility complex (MHC) class II molecules or to NK1.1+ T-cells via CD1 molecules. Cathepsin S also appears to participate in direct processing of exogenous antigens for presentation by MHC class II to CD4+ T-cells, or in cross-presentation by MHC class I molecules to CD8+ T-cells. In addition, although direct evidence is still lacking, in its secreted form cathepsin S is implicated in degradation of the extracellular matrix, which may contribute to the pathology of a number of diseases, including arthritis, atherosclerosis, neurological diseases and chronic obstructive pulmonary disease.

Description: A DNA sequence encoding the pro form of human CTSS (NP_004070.3) (Met 1-Ile 331) with a carboxy-terminal polyhistidine tag was expressed.

NCBI and Uniprot Product Information

• NCBI GI #: 23110962
• NCBI GeneID: 1520
• NCBI Accession #: NP_004070.3
• NCBI GenBank Nucleotide #: NM_004079.4
• UniProt Accession #: P25774; Q5T5I0; Q6FHS5; Q9BUG3; B4DWC9; D3DV05
• Molecular Weight: 31,626 Da
• NCBI Official Full Name: cathepsin S isoform 1 preproprotein
• NCBI Official Synonym Full Names: cathepsin S
• NCBI Official Symbol: CTSS
• NCBI Protein Information: cathepsin S
• UniProt Protein Name: Cathepsin S
• Protein Family: Cathepsin
• UniProt Gene Name: CTSS

NCBI Description

The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. The encoded protein can function as an elastase over a broad pH range in alveolar macrophages. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

CTSS: Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond- specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N. Belongs to the peptidase C1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.27; Protease

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: extracellular region; extracellular space; intracellular membrane-bound organelle; lysosomal lumen; lysosome

Molecular Function: collagen binding; cysteine-type endopeptidase activity; fibronectin binding; laminin binding; proteoglycan binding; serine-type endopeptidase activity

Biological Process: adaptive immune response; antigen processing and presentation; antigen processing and presentation of exogenous peptide antigen via MHC class II; antigen processing and presentation of peptide antigen; collagen catabolic process; extracellular matrix disassembly; immune response; protein processing; proteolysis; proteolysis involved in cellular protein catabolic process; response to acidity; toll-like receptor signaling pathway