Western Blot (WB)
(Western blot analysis of HSD17B2 Antibody (Center) in MDA-MB435 cell line lysates (35ug/lane).HSD17B2 (arrow) was detected using the purified Pab.)
Rabbit anti-Human HSD17B2 Polyclonal Antibody | anti-HSD17B2 antibody
HSD17B2, ID (HSD17B2, EDH17B2, Estradiol 17-beta-dehydrogenase 2, 17-beta-hydroxysteroid dehydrogenase type 2, 20 alpha-hydroxysteroid dehydrogenase, E2DH, Microsomal 17-beta-hydroxysteroid dehydrogenase, Testosterone 17-beta-dehydrogenase) (Azide free) (
Gene Names
HSD17B2; HSD17; SDR9C2; EDH17B2
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
HSD17B2; Polyclonal Antibody; ID (HSD17B2; EDH17B2; Estradiol 17-beta-dehydrogenase 2; 17-beta-hydroxysteroid dehydrogenase type 2; 20 alpha-hydroxysteroid dehydrogenase; E2DH; Microsomal 17-beta-hydroxysteroid dehydrogenase; Testosterone 17-beta-dehydrogenase) (Azide free) (; Testosterone 17-beta-dehydrogenase; anti-HSD17B2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-HSD17B2 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
HSD17B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-300 amino acids from the Central region of human HSD17B2.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of HSD17B2 Antibody (Center) in MDA-MB435 cell line lysates (35ug/lane).HSD17B2 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of HSD17B2 Antibody (Center) in MDA-MB435 cell line lysates (35ug/lane).HSD17B2 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(HSD17B2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HSD17B2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(HSD17B2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HSD17B2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(HSD17B2 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(HSD17B2 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-HSD17B2 antibody
HSD17B2 is capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone.HSD17B2 also has 20-alpha-HSD activity. HSD17B2 uses NADH while EDH17B3 uses NADPH.
Product Categories/Family for anti-HSD17B2 antibody
References
Shen, Z., et al. Endocrinology 150(11):4941-4949(2009). Bhavani, V., et al. Cancer Biomark 5(4):207-213(2009). Olsen, J.V., et al. Cell 127(3):635-648(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,785 Da
NCBI Official Full Name
estradiol 17-beta-dehydrogenase 2
NCBI Official Synonym Full Names
hydroxysteroid (17-beta) dehydrogenase 2
NCBI Official Symbol
HSD17B2
NCBI Official Synonym Symbols
HSD17; SDR9C2; EDH17B2
NCBI Protein Information
estradiol 17-beta-dehydrogenase 2; E2DH; 20-alpha-HSD; 17-beta-HSD 2; testosterone 17-beta-dehydrogenase; 20 alpha-hydroxysteroid dehydrogenase; 17-beta-hydroxysteroid dehydrogenase type 2; microsomal 17-beta-hydroxysteroid dehydrogenase; short chain dehy
UniProt Protein Name
Estradiol 17-beta-dehydrogenase 2
Protein Family
UniProt Gene Name
HSD17B2
UniProt Synonym Gene Names
EDH17B2; 17-beta-HSD 2
UniProt Entry Name
DHB2_HUMAN
Uniprot Description
HSD17B2: Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH. Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Protein type: EC 1.1.1.62; EC 1.1.1.239; Lipid Metabolism - androgen and estrogen; Endoplasmic reticulum; Membrane protein, integral; Oxidoreductase
Chromosomal Location of Human Ortholog: 16q24.1-q24.2
Cellular Component: endoplasmic reticulum membrane; integral to membrane
Molecular Function: estradiol 17-beta-dehydrogenase activity; 20-alpha-hydroxysteroid dehydrogenase activity; 3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity
Biological Process: response to retinoic acid; in utero embryonic development; steroid biosynthetic process; placenta development
Research Articles on HSD17B2
Similar Products
Product Notes
The HSD17B2 hsd17b2 (Catalog #AAA6308489) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSD17B2, ID (HSD17B2, EDH17B2, Estradiol 17-beta-dehydrogenase 2, 17-beta-hydroxysteroid dehydrogenase type 2, 20 alpha-hydroxysteroid dehydrogenase, E2DH, Microsomal 17-beta-hydroxysteroid dehydrogenase, Testosterone 17-beta-dehydrogenase) (Azide free) ( reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSD17B2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HSD17B2 hsd17b2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSD17B2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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