Goat PRDX1 / Peroxiredoxin 1 Polyclonal Antibody | anti-PRDX1 antibody

Goat Polyclonal to Human PRDX1 / Peroxiredoxin 1

Cat. Num. AAA244022

• Gene Names: PRDX1; PAG; PAGA; PAGB; PRX1; PRXI; MSP23; NKEFA; TDPX2; NKEF-A
• Reactivity: Bat, Bovine, Dog, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: PRDX1 / Peroxiredoxin 1; Polyclonal Antibody; Goat Polyclonal to Human PRDX1 / Peroxiredoxin 1; Anti-PRDX1 / Peroxiredoxin 1 Antibody (Internal) IHC-plus; PRDX1; MSP23; PAGB; Peroxiredoxin-1; PRX1; NKEFA; Peroxiredoxin 1; Thioredoxin peroxidase 2; NKEF-A; PAG; PRXI; TDPX2; Human PRDX1; anti-PRDX1 antibody

• Host: Goat
• Reactivity: Bat, Bovine, Dog, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat
• Clonality: Polyclonal
• Specificity: Human PRDX1 / Peroxiredoxin 1. Reported variants represent identical protein (NP_002565.1, NP_859047.1, NP_859048.1).
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: TBS, pH 7.3, 0.02% sodium azide, 0.5% BSA,
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Positions: 106-117
• Sequence Length: 117

• Applicable Applications for anti-PRDX1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:32000); IHC-P (2.5 ug/ml); WB (0.01 - 0.03 ug/ml); Peptide Enzyme-Linked Immunosorbent Assay (1:8000); ; Usage Summary: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx. 23 to 24kD band observed in lysates of cell lines HEK293, A431 and HepG2 (calculated MW of 22.1kD according to NP_859048.1). Recommended concentration: 0.01-0.03 ug/ml.
• Target: PRDX1 / Peroxiredoxin 1
• Epitope: aa106-117
• Immunogen: PRDX1 / Peroxiredoxin 1 antibody was raised against synthetic peptide C-SDPKRTIAQDYG from an internal region of human PRDX1 / Peroxiredoxin 1 (NP_859048.1).
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store at -20°C . Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-PRDX1 / Peroxiredoxin 1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-PRDX1 / Peroxiredoxin 1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

Western Blot (WB)

(Antibody (0.01 ug/ml) staining of HepG2 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 4505591
• NCBI GeneID: 5052
• NCBI Accession #: NP_002565.1
• NCBI GenBank Nucleotide #: NM_002574.3
• UniProt Accession #: Q06830; P35703; Q2V576; Q5T154; Q5T155; B5BU26; D3DPZ8
• NCBI Official Full Name: peroxiredoxin-1
• NCBI Official Synonym Full Names: peroxiredoxin 1
• NCBI Official Symbol: PRDX1
• NCBI Official Synonym Symbols: PAG; PAGA; PAGB; PRX1; PRXI; MSP23; NKEFA; TDPX2; NKEF-A
• NCBI Protein Information: peroxiredoxin-1; thioredoxin peroxidase 2; proliferation-associated gene A; natural killer-enhancing factor A; proliferation-associated gene protein; natural killer cell-enhancing factor A; thioredoxin-dependent peroxide reductase 2
• UniProt Protein Name: Peroxiredoxin-1
• Protein Family: Peroxiredoxin
• UniProt Gene Name: PRDX1
• UniProt Synonym Gene Names: PAGA; PAGB; TDPX2; NKEF-A; PAG
• UniProt Entry Name: PRDX1_HUMAN

NCBI Description

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Four transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

PRDX1: a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). May have a proliferative effect and play a role in cancer development or progression.

Protein type: EC 1.11.1.15; Oxidoreductase; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: extracellular space; peroxisomal matrix; mitochondrial matrix; cytoplasm; nucleolus; melanosome; nucleus; cytosol

Molecular Function: protein binding; peroxidase activity; protein homodimerization activity; thioredoxin peroxidase activity; heme binding

Biological Process: erythrocyte homeostasis; retinal homeostasis; cell proliferation; regulation of stress-activated MAPK cascade; response to reactive oxygen species; removal of superoxide radicals; hydrogen peroxide catabolic process; natural killer cell mediated cytotoxicity; skeletal development; regulation of NF-kappaB import into nucleus