Cardiotrophin 1 Recombinant Protein | CTF1 recombinant protein
Cardiotrophin 1, Human, Recombinant (CT1)
Gene Names
CTF1; CT1; CT-1
Applications
Western Blot
Purity
Highly PurifiedThree-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding. Purity of recombinant human cardiotrophin is >90% (SDS-PAGE analyzed).
Synonyms
Cardiotrophin 1; Human; Recombinant (CT1); CTF1 recombinant protein
Host
E Coli
Specificity
The amino acid sequence of the recombinant human cardiotrophin is 100% homologous to the amino acid sequence of the human cardiotrophin.
Purity/Purification
Highly Purified
Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding. Purity of recombinant human cardiotrophin is >90% (SDS-PAGE analyzed).
Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding. Purity of recombinant human cardiotrophin is >90% (SDS-PAGE analyzed).
Form/Format
Supplied as a lyophilized powder from 0.05M Acetate buffer pH4
Sequence
MRGSHHHHHH GSSRREGSLE DPQTDSSVSL LPHLEAKIRQ THSLAHLLTK YAEQLLQEYV QLQGDPFGLPSFSPPRLPVA GLSAPAPSHA GLPVHERLRL DAAALAALPP LLDAVCRRQA ELNPRAPRLL RRLEDAARQA RALGAAVEAL LAALGAANRG PRAEPPAATA SAASATGVFP AKVLGLRVCG LYREWLSRTE GDLGQLLPGG SA.
Applicable Applications for CTF1 recombinant protein
Western Blot (WB)
Reconstitution
Add 0.2ml of 0.1M Acetate buffer pH4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10ug/ml. In higher concentrations the solubility of this antigen is limited.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for CTF1 recombinant protein
Cardiotrophin-1 (CT-1) is a 201 amino acid member of the interleukin-6 superfamily. It was identified by its ability to induce hypertrophic response in cardiac myocytes.
Product Categories/Family for CTF1 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22.5kD
NCBI Official Full Name
cardiotrophin-1 isoform 1
NCBI Official Synonym Full Names
cardiotrophin 1
NCBI Official Symbol
CTF1
NCBI Official Synonym Symbols
CT1; CT-1
NCBI Protein Information
cardiotrophin-1; cardiophin 1; OTTHUMP00000163250
UniProt Protein Name
Cardiotrophin-1
Protein Family
UniProt Gene Name
CTF1
UniProt Entry Name
CTF1_HUMAN
NCBI Description
The protein encoded by this gene is a secreted cytokine that induces cardiac myocyte hypertrophy in vitro. It has been shown to bind and activate the ILST/gp130 receoptor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Uniprot Description
Function: Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor.
Subcellular location: Secreted.
Tissue specificity: Highly expressed in heart, skeletal muscle, prostate and ovary. Lower levels in lung, kidney, pancreas, thymus, testis and small intestine. Little or no expression in brain, placenta, liver, spleen, colon or peripheral blood leukocytes.
Sequence similarities: Belongs to the IL-6 superfamily.
Research Articles on CTF1
Similar Products
Product Notes
The CTF1 ctf1 (Catalog #AAA650767) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cardiotrophin 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the CTF1 ctf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MRGSHHHHHH GSSRREGSLE DPQTDSSVSL LPHLEAKIRQ THSLAHLLTK YAEQLLQEYV QLQGDPFGLP SFSPPRLPVA GLSAPAPSHA GLPVHERLRL DAAALAALPP LLDAVCRRQA ELNPRAPRLL RRLEDAARQA RALGAAVEAL LAALGAANRG PRAEPPAATA SAASATGVFP AKVLGLRVCG LYREWLSRTE GDLGQLLPGG SA. It is sometimes possible for the material contained within the vial of "Cardiotrophin 1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
