Typical Testing Data/Standard Curve (for reference only)
(Human Cardiotrophin-1 PicoKine ELISA Kit standard curve )
Human Cardiotrophin-1 ELISA Kit | CTF1 elisa kit
Human Cardiotrophin-1 PicoKine ELISA Kit
Gene Names
CTF1; CT1; CT-1
Reactivity
Human
Synonyms
Cardiotrophin-1; Human Cardiotrophin-1 PicoKine ELISA Kit; cardiotrophin 1; Cardiophin 1; Cardiophin1; Cardiotrophin I; CardiotrophinI; CT 1; CT-1; CT1; CTF 1; Ctf1; CTF1_HUMAN; CTF1 elisa kit
Reactivity
Human
Specificity
Natural and recombinant human CT-1
Sequence Length
201
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Human Cardiotrophin-1 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Human Cardiotrophin-1 PicoKine ELISA Kit standard curve )
Related Product Information for CTF1 elisa kit
Principle of the assay: human CT-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CT-1 has been precoated onto 96-well plates. Standards(E.coli, S2-A201) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CT-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CT-1 amount of sample captured in plate.
Background: Cardiotrophin-1 (CT-1) is a member of the family of cytokines that includes leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), oncostatin M (OSM), interleukin-6 (IL6), and interleukin-11 (IL11). And the CT-1 gene is mapped to 1p21-p13. The human CT-1 protein contains 201 amino acids and shares 80% amino acid identity with the 203-amino acid mouse CT-1 sequence; however, unlike the mouse protein, human CT-1 has 2 rather than 1 cys and has no N-glycosylation site. Despite lacking a signal sequence, secreted CT-1 and mouse CT-1 induce cardiac myocyte hypertrophy in cell culture and bind to both mouse and human LIFR but not to OSMR. Furthermore, A 1.7-kb CT-1 transcript was detected at high levels in heart, skeletal muscle, prostate, and ovary. Low levels were detected in lung, kidney, pancreas, thymus, testis, and small intestine, with little or no expression detected in brain, placenta, spleen, colon, and peripheral blood leukocytes. And it was also observed strong expression in fetal lung and kidney.
Background: Cardiotrophin-1 (CT-1) is a member of the family of cytokines that includes leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), oncostatin M (OSM), interleukin-6 (IL6), and interleukin-11 (IL11). And the CT-1 gene is mapped to 1p21-p13. The human CT-1 protein contains 201 amino acids and shares 80% amino acid identity with the 203-amino acid mouse CT-1 sequence; however, unlike the mouse protein, human CT-1 has 2 rather than 1 cys and has no N-glycosylation site. Despite lacking a signal sequence, secreted CT-1 and mouse CT-1 induce cardiac myocyte hypertrophy in cell culture and bind to both mouse and human LIFR but not to OSMR. Furthermore, A 1.7-kb CT-1 transcript was detected at high levels in heart, skeletal muscle, prostate, and ovary. Low levels were detected in lung, kidney, pancreas, thymus, testis, and small intestine, with little or no expression detected in brain, placenta, spleen, colon, and peripheral blood leukocytes. And it was also observed strong expression in fetal lung and kidney.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
21,098 Da
NCBI Official Full Name
Cardiotrophin-1
NCBI Official Synonym Full Names
cardiotrophin 1
NCBI Official Symbol
CTF1
NCBI Official Synonym Symbols
CT1; CT-1
NCBI Protein Information
cardiotrophin-1; cardiophin 1
UniProt Protein Name
Cardiotrophin-1
Protein Family
UniProt Gene Name
CTF1
UniProt Synonym Gene Names
CT-1
UniProt Entry Name
CTF1_HUMAN
NCBI Description
The protein encoded by this gene is a secreted cytokine that induces cardiac myocyte hypertrophy in vitro. It has been shown to bind and activate the ILST/gp130 receoptor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]
Uniprot Description
CTF1: Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytokine
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: extracellular space; extracellular region
Molecular Function: leukemia inhibitory factor receptor binding; cytokine activity
Biological Process: cell proliferation; nervous system development; muscle development; cell-cell signaling; leukemia inhibitory factor signaling pathway; positive regulation of cell proliferation; neuron development
Research Articles on CTF1
Similar Products
Product Notes
The Human CTF1 ctf1 (Catalog #AAA175862) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA175862 ELISA Kit recognizes Human CTF1. It is sometimes possible for the material contained within the vial of "Cardiotrophin-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
