CD40 Ligand Active Protein | CD40LG active protein

CD40 Ligand Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8122688

• Gene Names: Cd40lg; IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5; Tnlg8b
• Purity: >80% as determined by SDS-PAGE
• Synonyms: CD40 Ligand; CD40 Ligand Protein; Mouse; Recombinant (hFc Tag); Mouse CD40L/CD154/TNFSF5 Protein (Fc Tag); CD154 Protein; CD40-L Protein; Cd40l Protein; gp39 Protein; HIGM1 Protein; IGM Protein; IMD3 Protein; Ly-62 Protein; Ly62 Protein; T-BAM Protein; Tnfsf5 Protein; TRAP Protein; CD40 ligand; CD40LG active protein

• Host: HEK293 Cells
• Purity/Purification: >80% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gly115-Leu260

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu
• Tag: N-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the soluble form of mouse CD40LG (P27548)(Gly 115-Leu 260) was fused with the Fc region of human IgG1 at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse CD40 His at 2ug/ml (100uL/well) can bind Mouse CD40 Ligand hFc. The EC50 of Mouse CD40 Ligand hFc is 60-300ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized Mouse CD40 His at 2 ug/ml (100 ul/well) can bind Mouse CD40 Ligand hFc, the EC50 of Mouse CD40 Ligand hFc is 60-300 ng/mL.)

Related Product Information for CD40LG active protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD154, also known as CD40 ligand or CD40L, is a member of the TNF superfamily. While CD154 was originally found on T cell surface, its expression has since been found on a wide variety of cells, including platelets, mast cells, macrophages and NK cells. CD154's ability is achieved through binding to the CD40 on antigen-presenting cells (APC). In the macrophage cells, the primary signal for activation is IFN-gamma from Th1 type CD4 T cells. The secondary signal is CD40L on the T cell, which interacting with the CD40 molecules, helping increase the level of activation.

Product Categories/Family for CD40LG active protein

Cancer Drug Targets

References

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.Grewal IS, et al. (1998) CD40 and CD154 in cell-mediated immunity. Annual Review of Immunology. 16: 111-35.

NCBI and Uniprot Product Information

• NCBI GI #: 15011846
• NCBI GeneID: 21947
• NCBI Accession #: NP_035746.2
• NCBI GenBank Nucleotide #: NM_011616.2
• UniProt Accession #: P27548
• Molecular Weight: 29,370 Da
• NCBI Official Full Name: CD40 ligand
• NCBI Official Synonym Full Names: CD40 ligand
• NCBI Official Symbol: Cd40lg
• NCBI Official Synonym Symbols: IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5; Tnlg8b
• NCBI Protein Information: CD40 ligand
• UniProt Protein Name: CD40 ligand
• Protein Family: CD40 ligand
• UniProt Gene Name: Cd40lg
• UniProt Synonym Gene Names: Cd40l; Tnfsf5; CD40-L; TRAP
• UniProt Entry Name: CD40L_MOUSE

Uniprot Description

CD40LG: Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1); also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. Belongs to the tumor necrosis factor family.

Protein type: Cell adhesion; Membrane protein, integral

Cellular Component: cell projection; cell surface; external side of plasma membrane; extracellular space

Molecular Function: CD40 receptor binding

Biological Process: activation of JNK activity; activation of NF-kappaB transcription factor; B cell differentiation; B cell proliferation; immunoglobulin secretion; inflammatory response; isotype switching; negative regulation of apoptosis; platelet activation; positive regulation of B cell proliferation; positive regulation of immune response to tumor cell; positive regulation of interferon-gamma production; positive regulation of interleukin-10 production; positive regulation of interleukin-12 production; positive regulation of interleukin-4 production; positive regulation of MHC class II biosynthetic process; positive regulation of T cell proliferation; regulation of immunoglobulin secretion