ITGA8 & ITGB1 Heterodimer Recombinant Protein | ITGA8 recombinant protein

Recombinant Human ITGA8 & ITGB1 Heterodimer Protein

Cat. Num. AAA2545178

• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: ITGA8 & ITGB1 Heterodimer; Recombinant Human ITGA8 & ITGB1 Heterodimer Protein; ITGA8; RP11-479G22.2; CD29; FNRB; GPIIA; MDF2; MSK12; VLA-BETA; VLAB; ITGA8 recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 1063

• Application Notes: The recombinant heterodimer of human ITGA8/ITGB1 comprises 1765 (1016 + 749) amino acids and has a calculated molecular mass of 196 (113 + 83) kDa. As a result of glycosylation, the apparent molecular mass of rh ITGA8/ITGB1 heterodimer is approximately 120 & 140 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Phe 39 & Gln 21
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for ITGA8 recombinant protein

A DNA sequence encoding the extracellular domain (Met 1-Leu 1012) of human ITGA8 (P53708) was fused with a DYKDDDDK tag at the C-terminus, constructed the plasmid 1 ; A DNA sequence encoding the extracellular domain (Met 1-Asp 728) of human ITGB1 (P05556-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified

NCBI and Uniprot Product Information

• NCBI GI #: 612407851
• NCBI GeneID: 8516
• NCBI Accession #: NP_003629.2
• NCBI GenBank Nucleotide #: NM_003638.2
• UniProt Accession #: P53708; Q5VX94; B0YJ31
• Molecular Weight: 117,474 Da
• NCBI Official Full Name: integrin alpha-8 Isoform 1 preproprotein
• NCBI Official Synonym Full Names: integrin subunit alpha 8
• NCBI Official Symbol: ITGA8
• NCBI Protein Information: integrin alpha-8
• UniProt Protein Name: Integrin alpha-8
• Protein Family: Integrin
• UniProt Gene Name: ITGA8

NCBI Description

Integrins are heterodimeric transmembrane receptor proteins that mediate numerous cellular processes including cell adhesion, cytoskeletal rearrangement, and activation of cell signaling pathways. Integrins are composed of alpha and beta subunits. This gene encodes the alpha 8 subunit of the heterodimeric integrin alpha8beta1 protein. The encoded protein is a single-pass type 1 membrane protein that contains multiple FG-GAP repeats. This repeat is predicted to fold into a beta propeller structure. This gene regulates the recruitment of mesenchymal cells into epithelial structures, mediates cell-cell interactions, and regulates neurite outgrowth of sensory and motor neurons. The integrin alpha8beta1 protein thus plays an important role in wound-healing and organogenesis. Mutations in this gene have been associated with renal hypodysplasia/aplasia-1 (RHDA1) and with several animal models of chronic kidney disease. Alternate splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Apr 2014]

Uniprot Description

ITGA8: Integrin alpha-8/beta-1 functions in the genesis of kidney and probably of other organs by regulating the recruitment of mesenchymal cells into epithelial structures. It recognizes the sequence R-G-D in a wide array of ligands including TNC, FN1, SPP1 TGFB1, TGFB3 and VTN. NPNT is probably its functional ligand in kidney genesis. Neuronal receptor for TNC it mediates cell-cell interactions and regulates neurite outgrowth of sensory and motor neurons. Belongs to the integrin alpha chain family.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: cell surface; endoplasmic reticulum; focal adhesion; integrin complex; plasma membrane

Biological Process: cell-matrix adhesion; extracellular matrix organization and biogenesis; kidney development; mesodermal cell differentiation

Disease: Renal Hypodysplasia/aplasia 1