Cystatin-C Recombinant Protein | CST3 recombinant protein

Recombinant Mouse Cystatin-C, sf9

Cat. Num. AAA140963

• Gene Names: Cst3; CysC
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Synonyms: Cystatin-C; Recombinant Mouse Cystatin-C; sf9; CST3 Mouse; Cystatin-C Mouse Recombinant; Cystatin-3; Cst3; CST3 recombinant protein

• Host: Sf9 Insect Cells
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE.
• Form/Format: CST3 protein solution (1mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.; Sterile filtered colorless solution.
• Sequence: ATPKQGPRMLGAPEEADANE EGVRRALDFA VSEYNKGSND AYHSRAIQVV RARKQLVAGV NYFLDVEMGR TTCTKSQTNLTDCPFHDQPH LMRKALCSFQ IYSVPWKGTH SLTKFSCKNA HHHHHH.
• Sequence Length: 140

Related Product Information for CST3 recombinant protein

Introduction: Cystatins are a superfamily of cysteine proteinase inhibitors found in both plants and animals. They comprise a group of proteinase inhibitors, widely distributed in tissues and body fluids, and form tight complexes with cysteine proteases such as cathepsin B, H, L and S. Cystatin C, a secreted molecule of this family, is of interest from biochemical, medicine and evolutionary points of view. Cystatin C, with molecular weight of 13260 Da, is composed of 120 amino acids, lacks carbohydrate and has two disulfide bridges located near the carboxyl terminus. Cystatin C is increased in patients with malignant diseases, and is related to the insufficiency of renal function and appears to be a better marker than creatinine. On the other hand, low levels of cystatin C involve cause the breakdown of the elastic laminae and, subsequently, the atherosclerosis and abdominal aortic aneurysm.

Description: CST3 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 126 amino acids (21-140a.a.) and having a molecular mass of 14.2kDa. (Molecular size on SDS-PAGE will appear at approximately 13.5-18kDa).CST3 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Categories/Family for CST3 recombinant protein

Recombinant & Natural Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 31981822
• NCBI GeneID: 13010
• NCBI Accession #: NP_034106.2
• NCBI GenBank Nucleotide #: NM_009976.4
• UniProt Accession #: P21460; Q544Y0
• Molecular Weight: 15,531 Da
• NCBI Official Full Name: cystatin-C
• NCBI Official Synonym Full Names: cystatin C
• NCBI Official Symbol: Cst3
• NCBI Official Synonym Symbols: CysC
• NCBI Protein Information: cystatin-C
• UniProt Protein Name: Cystatin-C
• Protein Family: Cystatin
• UniProt Gene Name: Cst3
• UniProt Entry Name: CYTC_MOUSE

Uniprot Description

CST3: As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Defects in CST3 are the cause of amyloidosis type 6 (AMYL6); also known as hereditary cerebral hemorrhage with amyloidosis (HCHWA), cerebral amyloid angiopathy (CAA) or cerebroarterial amyloidosis Icelandic type. AMYL6 is a hereditary generalized amyloidosis due to cystatin C amyloid deposition. Cystatin C amyloid accumulates in the walls of arteries, arterioles, and sometimes capillaries and veins of the brain, and in various organs including lymphoid tissue, spleen, salivary glands, and seminal vesicles. Amyloid deposition in the cerebral vessels results in cerebral amyloid angiopathy, cerebral hemorrhage and premature stroke. Cystatin C levels in the cerebrospinal fluid are abnormally low. Genetic variations in CST3 are associated with age- related macular degeneration type 11 (ARMD11). ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. Belongs to the cystatin family.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Cellular Component: axon; basement membrane; cell projection; cell soma; contractile fiber; cytoplasm; endoplasmic reticulum; extracellular region; extracellular space; lysosome; multivesicular body; nuclear membrane; perinuclear region of cytoplasm; vesicle

Molecular Function: beta-amyloid binding; cysteine protease inhibitor activity; endopeptidase inhibitor activity; identical protein binding; protease binding; protease inhibitor activity

Biological Process: defense response; fibril organization and biogenesis; negative regulation of peptidase activity; negative regulation of proteolysis; positive regulation of cell proliferation; positive regulation of DNA replication; regulation of programmed cell death; response to oxidative stress