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Western Blot (WB) (CHST9 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CHST9 antibody detected the CHST9 protein (arrow).)

Rabbit anti-Human CHST9 Polyclonal Antibody | anti-CHST9 antibody

CHST9, NT (CHST9, Carbohydrate sulfotransferase 9, GalNAc-4-O-sulfotransferase 2, N-acetylgalactosamine-4-O-sulfotransferase 2) (AP)

Gene Names
CHST9; GalNAc4ST2; GALNAC4ST-2
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
CHST9; Polyclonal Antibody; NT (CHST9; Carbohydrate sulfotransferase 9; GalNAc-4-O-sulfotransferase 2; N-acetylgalactosamine-4-O-sulfotransferase 2) (AP); N-acetylgalactosamine-4-O-sulfotransferase 2; anti-CHST9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Sequence Length
443
Applicable Applications for anti-CHST9 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
CHST9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-50 amino acids from the N-terminal region of human CHST9.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CHST9 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CHST9 antibody detected the CHST9 protein (arrow).)

Western Blot (WB) (CHST9 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CHST9 antibody detected the CHST9 protein (arrow).)
Related Product Information for anti-CHST9 antibody
Sulfate groups in carbohydrates confer highly specific functions on glycoproteins, glycolipids, and proteoglycans and are critical for cell-cell interaction, signal transduction, and embryonic development. Sulfotransferases, such as CHST9, carry out sulfation of carbohydrates (Hiraoka et al., 2001 [PubMed 11445554]).
Product Categories/Family for anti-CHST9 antibody
References
Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003). Hiraoka, N., et al. Glycobiology 11(6):495-504(2001). Kang, H.G., et al. J. Biol. Chem. 276(14):10861-10869(2001).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
carbohydrate sulfotransferase 9 isoform 1
NCBI Official Synonym Full Names
carbohydrate sulfotransferase 9
NCBI Official Symbol
CHST9
NCBI Official Synonym Symbols
GalNAc4ST2; GALNAC4ST-2
NCBI Protein Information
carbohydrate sulfotransferase 9
UniProt Protein Name
Carbohydrate sulfotransferase 9
UniProt Gene Name
CHST9
UniProt Synonym Gene Names
GalNAc-4-ST2; GalNAc4ST-2
UniProt Entry Name
CHST9_HUMAN

NCBI Description

The protein encoded by this gene belongs to the sulfotransferase 2 family. It is localized to the golgi membrane, and catalyzes the transfer of sulfate to position 4 of non-reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. Sulfate groups on carbohydrates confer highly specific functions to glycoproteins, glycolipids, and proteoglycans, and are critical for cell-cell interaction, signal transduction, and embryonic development. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

CHST9: Catalyzes the transfer of sulfate to position 4 of non- reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. Participates in biosynthesis of glycoprotein hormones lutropin and thyrotropin, by mediating sulfation of their carbohydrate structures. Has a higher activity toward carbonic anhydrase VI than toward lutropin. Only active against terminal GalNAcbeta1,GalNAcbeta. Isoform 2, but not isoform 1, is active toward chondroitin. Belongs to the sulfotransferase 2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 2.8.2.-; Transferase

Chromosomal Location of Human Ortholog: 18q11.2

Cellular Component: Golgi membrane; integral to membrane; extracellular region

Molecular Function: N-acetylgalactosamine 4-O-sulfotransferase activity

Biological Process: chondroitin sulfate metabolic process; sulfur metabolic process; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; proteoglycan biosynthetic process; carbohydrate metabolic process; pathogenesis; hormone biosynthetic process; carbohydrate biosynthetic process

Research Articles on CHST9

Similar Products

Product Notes

The CHST9 chst9 (Catalog #AAA6285997) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHST9, NT (CHST9, Carbohydrate sulfotransferase 9, GalNAc-4-O-sulfotransferase 2, N-acetylgalactosamine-4-O-sulfotransferase 2) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHST9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CHST9 chst9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHST9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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