Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Typical Testing Data/Standard Curve (for reference only)

Mouse Cystatin C ELISA Kit | CST3 elisa kit

Mouse Cystatin C PicoKine ELISA Kit

Gene Names
Cst3; CysC
Reactivity
Mouse
Synonyms
Cystatin C; Mouse Cystatin C PicoKine ELISA Kit; Cystatin-C; cystatin C; Cst3; Cystatin-3; CYTC_HUMAN; Gamma-trace; Neuroendocrine basic polypeptide; Post-gamma-globulin; CST3 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Cst3
Sequence Length
140
Samples
Cell culture supernatants, serum, plasma (heparin, edta) and urine
Detection Range
312 pg/ml - 20,000 pg/ml
Sensitivity
<10 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CST3 elisa kit
Principle of the Assay: The Picokine™ Mouse Cst3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Cst3 with a 96-well strip plate that is pre-coated with antibody specific for Cst3. The detection antibody is a biotinylated antibody specific for Cst3. The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Cst3 with immunogen: Expression system for standard: NS0; Immunogen sequence: M1-A140. The kit is analytically validated with ready-to-use reagents. To measure Mouse Cst3, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Cst3 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Cst3 in the sample.

Background/Introduction: Cystatin C or cystatin 3 (formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid (CSF) and of urine from patients with renal failure. Cystatin 3 has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In mouses, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
15,531 Da
NCBI Official Full Name
Cystatin-C
NCBI Official Synonym Full Names
cystatin C
NCBI Official Symbol
Cst3
NCBI Official Synonym Symbols
CysC
NCBI Protein Information
cystatin-C; cystatin 3; cystatin-3
UniProt Protein Name
Cystatin-C
Protein Family
UniProt Gene Name
Cst3
UniProt Entry Name
CYTC_MOUSE

NCBI Description

The protein encoded by this gene is a cysteine protease inhibitor involved in neurodegenerative and cardiovascular processes. The encoded protein inhibits aggregation of beta-amyloid protein, a hallmark of Alzheimer's disease, so it may be useful as a therapeutic. This protein also may be a biomarker for atherosclerosis. [provided by RefSeq, Aug 2015]

Uniprot Description

CST3: As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Defects in CST3 are the cause of amyloidosis type 6 (AMYL6); also known as hereditary cerebral hemorrhage with amyloidosis (HCHWA), cerebral amyloid angiopathy (CAA) or cerebroarterial amyloidosis Icelandic type. AMYL6 is a hereditary generalized amyloidosis due to cystatin C amyloid deposition. Cystatin C amyloid accumulates in the walls of arteries, arterioles, and sometimes capillaries and veins of the brain, and in various organs including lymphoid tissue, spleen, salivary glands, and seminal vesicles. Amyloid deposition in the cerebral vessels results in cerebral amyloid angiopathy, cerebral hemorrhage and premature stroke. Cystatin C levels in the cerebrospinal fluid are abnormally low. Genetic variations in CST3 are associated with age- related macular degeneration type 11 (ARMD11). ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. Belongs to the cystatin family.

Protein type: Secreted, signal peptide; Secreted; Inhibitor

Cellular Component: multivesicular body; extracellular space; nuclear membrane; cell projection; contractile fiber; cell soma; perinuclear region of cytoplasm; lysosome; axon; endoplasmic reticulum; cytoplasm; extracellular region; basement membrane; vesicle

Molecular Function: protease binding; protease inhibitor activity; beta-amyloid binding; endopeptidase inhibitor activity; cysteine protease inhibitor activity

Biological Process: negative regulation of proteolysis; positive regulation of cell proliferation; fibril organization and biogenesis; negative regulation of peptidase activity; defense response; response to oxidative stress; regulation of programmed cell death; positive regulation of DNA replication

Research Articles on CST3

Similar Products

Product Notes

The Mouse CST3 cst3 (Catalog #AAA175999) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA175999 ELISA Kit recognizes Mouse CST3. It is sometimes possible for the material contained within the vial of "Cystatin C, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.