Rabbit Coronin 1a Polyclonal Antibody | anti-CORO1A antibody

Rabbit Polyclonal to Coronin 1a Antibody

Cat. Num. AAA415601

• Gene Names: CORO1A; p57; IMD8; TACO; CLABP; HCORO1; CLIPINA
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot
• Purity: Affinity purified
• Synonyms: Coronin 1a; Polyclonal Antibody; Rabbit Polyclonal to Coronin 1a Antibody; Rabbit Polyclonal to Coronin 1a; anti-CORO1A antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 461

• Applicable Applications for anti-CORO1A antibody: Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Try at dilutions of 1:50 to 1:100 for immunofluorescence, and 1:500 for ABC or other enzyme linked immunocytochemical procedures. For western blots try at 1:2000.
• Immunogen: Our antibody was made against full length recombinant Coronin 1a expressed in and purified from E. coli.
• Storage Buffer: PBS, pH 7.4 with 0.02% sodium azide.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB) #2

Testing Data

Related Product Information for anti-CORO1A antibody

Rabbit Polyclonal to Coronin 1a is a Coronin 1a belongs to the WD repeat coronin family and contains 5 WD repeats. It may be an essential component of the cytoskeleton of highly motile cells, with roles both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In cell infected by mycobacteria, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes. In the CNS, coronin 1A is expressed exclusively by microglial cells. Although its function is not entirely clear, it may act as a linkage protein between the actin cytoskeleton and the plasma membrane, and perhaps the extracellular matrix.

NCBI and Uniprot Product Information

• NCBI GI #: 300934762
• NCBI GeneID: 11151
• NCBI Accession #: NP_001180262.1
• NCBI GenBank Nucleotide #: NM_001193333.2
• UniProt Accession #: P31146; Q2YD73; B2RBL1
• Molecular Weight: 51,026 Da
• NCBI Official Full Name: coronin-1A
• NCBI Official Synonym Full Names: coronin, actin binding protein, 1A
• NCBI Official Symbol: CORO1A
• NCBI Official Synonym Symbols: p57; IMD8; TACO; CLABP; HCORO1; CLIPINA
• NCBI Protein Information: coronin-1A; clipin-A; coronin-like protein A; coronin-like protein p57; tryptophan aspartate-containing coat protein
• UniProt Protein Name: Coronin-1A
• Protein Family: Coronin
• UniProt Gene Name: CORO1A
• UniProt Synonym Gene Names: CORO1; Clipin-A; TACO
• UniProt Entry Name: COR1A_HUMAN

NCBI Description

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16. [provided by RefSeq, Sep 2010]

Uniprot Description

Function: May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria-infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes. Ref.10

Subunit structure: Binds actin.

Subcellular location: Cytoplasm › cytoskeleton

By similarity. Cytoplasm › cell cortex

By similarity. Cytoplasmic vesicle › phagosome membrane

By similarity. Note: In non-infected macrophages, associated with the cortical microtubule network. In mycobacteria-infected macrophages, becomes progressively relocalized and retained around the mycobacterial phagosomes. Retention on the phagosomal membrane is strictly dependent on mycobacterial viability and not due to impaired acidification

By similarity.

Tissue specificity: Expressed in brain, thymus, spleen, bone marrow and lymph node. Low in lung and gut.

Post-translational modification: phosphorylation at Thr-412 by PKC strongly down-regulates the association with actin.

Sequence similarities: Belongs to the WD repeat coronin family.Contains 7 WD repeats.