Rat cystatin C ELISA Kit | CST3 elisa kit

Rat Cystatin C, Cys-C ELISA Kit

Cat. Num. AAA704068

• Reactivity: Rat
• Synonyms: cystatin C; Rat Cystatin C; Cys-C ELISA Kit; ARMD11; MGC117328; OTTHUMP00000164181; OTTHUMP00000164182; bA218C14.4 (cystatin C) ; cystatin 3; gamma-trace; neuroendocrine basic polypeptide; post-gamma-globulin; CST3 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat CST3. No significant cross-reactivity or interference between Rat CST3 and analogues was observed.
• Sequence Length: 148

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Detection Range: 7.8 ng/ml-500 ng/ml
• Sensitivity: 1.95 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CST3 elisa kit

Introduction: CystatinC or cystatin 3 (formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, is mainly used as a biomarker of kidney function. Recently, it has been studied for its role in predicting new-onset or deteriorating cardiovascular disease. It also seems to play a role in brain disorders involving amyloid (a specific type of protein deposition), such as Alzheimer's disease. In humans, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. It is a potent inhibitor of lysosomal proteinases (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Cystatin C belongs to the type 2 cystatin gene family.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cys-C. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Cys-C and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Cys-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Cys-C in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 130485692
• NCBI GeneID: 100009450
• NCBI Accession #: NP_001076175.1
• NCBI GenBank Nucleotide #: NM_001082706.1
• UniProt Accession #: O97862
• Molecular Weight: 16,346 Da
• NCBI Official Full Name: cystatin-C
• NCBI Official Symbol: CST3
• NCBI Protein Information: cystatin-C; cystatin-3
• UniProt Protein Name: Cystatin-C
• Protein Family: Cystatin
• UniProt Gene Name: CST3
• UniProt Entry Name: CYTC_RABIT

Uniprot Description

Function: This is a thiol proteinase inhibitor.