Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Regulator Of G Protein Signaling 9 Polyclonal Antibody | anti-RGS9 antibody
Polyclonal Antibody to Regulator Of G Protein Signaling 9 (RGS9)
Gene Names
Rgs9; RGS9-1; Rgs9-2
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Regulator Of G Protein Signaling 9; Polyclonal Antibody; Polyclonal Antibody to Regulator Of G Protein Signaling 9 (RGS9); PERRS; RGS9L; anti-RGS9 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against RGS9. It has been selected for its ability to recognize RGS9 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence Length
443
Applicable Applications for anti-RGS9 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Fragment
RGS9 (Met1~Trp296)
Conjugate
No Conjugate
Cross Reactivity
Mouse
Immunoglobulin Type
IgG
Quality Control
Content: The quality control contains recombinant RGS9 (Met1~Trp296) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2072578
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded brain tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded brain tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56,940 Da
NCBI Official Full Name
regulator of G-protein signaling 9 isoform 1
NCBI Official Synonym Full Names
regulator of G-protein signaling 9
NCBI Official Symbol
Rgs9
NCBI Official Synonym Symbols
RGS9-1; Rgs9-2
NCBI Protein Information
regulator of G-protein signaling 9
UniProt Protein Name
Regulator of G-protein signaling 9
Protein Family
UniProt Gene Name
Rgs9
UniProt Synonym Gene Names
RGS9
Uniprot Description
RGS9: belongs to the 'regulator of G protein signaling' family. Inhibits signal transduction by increasing the GTPAse activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(t)-alpha. Involved in phototransduction; key element in the recovery phase of visual transduction. Four alternatively spliced isoforms have been described.
Protein type: GAP; GAP, RGS
Chromosomal Location of Human Ortholog: 11 E1|11 71.86 cM
Cellular Component: cytoplasm; heterotrimeric G-protein complex; membrane; nucleus; photoreceptor inner segment; photoreceptor outer segment; plasma membrane
Molecular Function: GTPase activator activity; protein binding; protein complex binding; signal transducer activity
Biological Process: dopamine receptor signaling pathway; G-protein coupled receptor signaling pathway; negative regulation of signal transduction; positive regulation of GTPase activity; regulation of G-protein coupled receptor protein signaling pathway; response to stimulus; visual perception
Research Articles on RGS9
Similar Products
Product Notes
The RGS9 rgs9 (Catalog #AAA2034008) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Regulator Of G Protein Signaling 9 (RGS9) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Regulator Of G Protein Signaling 9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-1:5000. Researchers should empirically determine the suitability of the RGS9 rgs9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Regulator Of G Protein Signaling 9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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