Sequence Information
N-Acetyl Beta-D-Glucosaminidase (NAGase) Recombinant Protein | NAGase recombinant protein
Recombinant N-Acetyl Beta-D-Glucosaminidase (NAGase)
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Sequence Information
Sequence Information
Gene Sequencing (extract)
Gene Sequencing (extract)
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
NCBI Description
The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM, Mar 2008]
Uniprot Description
OGA: Cleaves GlcNAc but not GalNAc from glycopeptides. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl- beta-GalNAc or p-nitrophenyl-alpha-GlcNAc. Possesses hyaluronidase activity. Acetylates 'Lys-8' of histone H4 and 'Lys-14' of histone H3. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.2.1.169; Acetyltransferase; EC 3.2.1.52; Hydrolase
Chromosomal Location of Human Ortholog: 10q24.1-q24.3
Cellular Component: membrane; cytosol; nucleus
Molecular Function: histone acetyltransferase activity; beta-N-acetylglucosaminidase activity; hyalurononglucosaminidase activity
Biological Process: positive regulation of DNA metabolic process; positive regulation of protein complex disassembly; dATP metabolic process; positive regulation of proteolysis; protein deglycosylation; N-acetylglucosamine metabolic process; positive regulation of insulin secretion; positive regulation of growth hormone secretion; positive regulation of glucose import; negative regulation of protein amino acid glycosylation; positive regulation of mitochondrial depolarization; response to steroid hormone stimulus; positive regulation of cell killing; histone acetylation; glycoprotein catabolic process; protein targeting to membrane; aging
Research Articles on NAGase
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Product Notes
The NAGase mgea5 (Catalog #AAA2012338) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant N-Acetyl Beta-D-Glucosaminidase (NAGase) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's N-Acetyl Beta-D-Glucosaminidase (NAGase) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the NAGase mgea5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "N-Acetyl Beta-D-Glucosaminidase (NAGase), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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