Human Corticotropin-releasing factor-binding protein ELISA Kit | CRHBP elisa kit

Human Corticotropin-releasing factor-binding protein ELISA Kit

Cat. Num. AAA764069

• Gene Names: CRHBP; CRFBP; CRF-BP
• Reactivity: Human
• Synonyms: Corticotropin-releasing factor-binding protein; Human Corticotropin-releasing factor-binding protein ELISA Kit; CRHBP/Corticotropin-releasing factor-binding protein/CRFBP/corticotropin releasing hormone binding protein/CRFBPcorticotropin releasing hormone-binding protein/CRF-BPCRF-binding protein/CRH-BP.; CRHBP elisa kit

• Reactivity: Human
• Sequence Length: 322

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CRHBP elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human CRHBP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human CRHBP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CRHBP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human CRHBP can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 47080099
• NCBI GeneID: 1393
• NCBI Accession #: NP_001873.2
• NCBI GenBank Nucleotide #: NM_001882.3
• Molecular Weight: 36,144 Da
• NCBI Official Full Name: corticotropin-releasing factor-binding protein
• NCBI Official Synonym Full Names: corticotropin releasing hormone binding protein
• NCBI Official Symbol: CRHBP
• NCBI Official Synonym Symbols: CRFBP; CRF-BP
• NCBI Protein Information: corticotropin-releasing factor-binding protein
• UniProt Protein Name: Corticotropin-releasing factor-binding protein
• Protein Family: Corticotropin-releasing factor-binding protein
• UniProt Gene Name: CRHBP
• UniProt Synonym Gene Names: CRFBP; CRF-BP; CRF-binding protein; CRH-BP
• UniProt Entry Name: CRHBP_HUMAN

NCBI Description

Corticotropin-releasing hormone is a potent stimulator of synthesis and secretion of preopiomelanocortin-derived peptides. Although CRH concentrations in the human peripheral circulation are normally low, they increase throughout pregnancy and fall rapidly after parturition. Maternal plasma CRH probably originates from the placenta. Human plasma contains a CRH-binding protein which inactivates CRH and which may prevent inappropriate pituitary-adrenal stimulation in pregnancy. [provided by RefSeq, Jul 2008]

Uniprot Description

CRHBP: Binds CRF and inactivates it. May prevent inappropriate pituitary-adrenal stimulation in pregnancy. Belongs to the CRF-binding protein family.

Protein type: Secreted, signal peptide; Secreted; Inhibitor

Chromosomal Location of Human Ortholog: 5q13.3

Cellular Component: dendrite; endoplasmic reticulum; extracellular space; Golgi apparatus; intracellular; microtubule; multivesicular body; nerve terminal; nucleus; perikaryon; secretory granule

Molecular Function: corticotropin-releasing hormone binding; peptide binding; protein binding

Biological Process: behavioral response to ethanol; cellular response to stress; female pregnancy; hormone metabolic process; hormone-mediated signaling; inflammatory response; learning and/or memory; negative regulation of adrenocorticotropic hormone secretion; regulated secretory pathway; regulation of adrenocorticotropin hormone secretion; signal transduction; synaptic transmission, dopaminergic