Human CRHBP ELISA Kit | CRHBP elisa kit

Human CRHBP (Corticotropin Releasing Hormone Bingding Protein) ELISA Kit

Cat. Num. AAA2501555

• Gene Names: CRHBP; CRFBP; CRF-BP
• Reactivity: Human
• Synonyms: CRHBP; Human CRHBP (Corticotropin Releasing Hormone Bingding Protein) ELISA Kit; CRHBP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human CRHBP. No significant cross-reactivity or interference between Human CRHBP and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human CRHBP and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 322

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CRHBP elisa kit

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CRHBP in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CRHBP. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human CRHBP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CRHBP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CRHBP. You can calculate the concentration of Human CRHBP in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 47080099
• NCBI GeneID: 1393
• NCBI Accession #: NP_001873.2
• NCBI GenBank Nucleotide #: NM_001882.3
• UniProt Accession #: P24387; Q53F32; Q6FHT5
• Molecular Weight: 36,144 Da
• NCBI Official Full Name: corticotropin-releasing factor-binding protein
• NCBI Official Synonym Full Names: corticotropin releasing hormone binding protein
• NCBI Official Symbol: CRHBP
• NCBI Official Synonym Symbols: CRFBP; CRF-BP
• NCBI Protein Information: corticotropin-releasing factor-binding protein; CRF-binding protein; CRH-BP; corticotropin releasing hormone-binding protein; corticotropin-releasing hormone-binding protein
• UniProt Protein Name: Corticotropin-releasing factor-binding protein
• Protein Family: Corticotropin-releasing factor-binding protein
• UniProt Gene Name: CRHBP
• UniProt Synonym Gene Names: CRFBP; CRF-BP; CRF-binding protein; CRH-BP
• UniProt Entry Name: CRHBP_HUMAN

NCBI Description

Corticotropin-releasing hormone is a potent stimulator of synthesis and secretion of preopiomelanocortin-derived peptides. Although CRH concentrations in the human peripheral circulation are normally low, they increase throughout pregnancy and fall rapidly after parturition. Maternal plasma CRH probably originates from the placenta. Human plasma contains a CRH-binding protein which inactivates CRH and which may prevent inappropriate pituitary-adrenal stimulation in pregnancy. [provided by RefSeq, Jul 2008]

Uniprot Description

CRHBP: Binds CRF and inactivates it. May prevent inappropriate pituitary-adrenal stimulation in pregnancy. Belongs to the CRF-binding protein family.

Protein type: Secreted; Secreted, signal peptide; Inhibitor

Chromosomal Location of Human Ortholog: 5q13.3

Cellular Component: multivesicular body; Golgi apparatus; extracellular space; microtubule; endoplasmic reticulum; dendrite; perikaryon; intracellular; nerve terminal; nucleus; secretory granule

Molecular Function: protein binding; corticotropin-releasing hormone binding; peptide binding

Biological Process: negative regulation of adrenocorticotropic hormone secretion; synaptic transmission, dopaminergic; regulated secretory pathway; learning and/or memory; hormone-mediated signaling; cellular response to stress; regulation of adrenocorticotropin hormone secretion; hormone metabolic process; behavioral response to ethanol; female pregnancy; inflammatory response; signal transduction