Rabbit anti-Mouse DOK7 Polyclonal Antibody | anti-DOK7 antibody

DOK7, NT (DOK7, C4orf25, Protein Dok-7, Downstream of tyrosine kinase 7)

Cat. Num. AAA6009026

• Gene Names: DOK7; CMS1B; C4orf25
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: DOK7; Polyclonal Antibody; NT (DOK7; C4orf25; Protein Dok-7; Downstream of tyrosine kinase 7); Anti -DOK7; anti-DOK7 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-DOK7 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: DOK7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-55 amino acids from the N-terminal region of human DOK7.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse spleen tissue lysates (35ug/lane) using MBS6009026. This demonstrates that MBS6009026 detected the DOK7 protein (arrow).)

Related Product Information for anti-DOK7 antibody

The protein encoded by this gene is essential for neuromuscular synaptogenesis. The protein functions in aneural activation of muscle-specific receptor kinase, which is required for postsynaptic differentiation, and in the subsequent clustering of the acetylcholine receptor in myotubes. This protein can also induce autophosphorylation of muscle-specific receptor kinase. Mutations in this gene are a cause of familial limb-girdle myasthenia autosomal recessive, which is also known as congenital myasthenic syndrome type 1B. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-DOK7 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 38566143
• NCBI GeneID: 285489
• UniProt Accession #: Q18PE1; Q6P6A6; Q86XG5; Q8N2J3; Q8NBC1; A2A499; A2RRD4; E9PB56
• Molecular Weight: 53,097 Da
• NCBI Official Full Name: DOK7 protein
• NCBI Official Synonym Full Names: docking protein 7
• NCBI Official Symbol: DOK7
• NCBI Official Synonym Symbols: CMS1B; C4orf25
• NCBI Protein Information: protein Dok-7; downstream of tyrosine kinase 7
• UniProt Protein Name: Protein Dok-7
• Protein Family: Protein
• UniProt Gene Name: DOK7
• UniProt Synonym Gene Names: C4orf25
• UniProt Entry Name: DOK7_HUMAN

NCBI Description

The protein encoded by this gene is essential for neuromuscular synaptogenesis. The protein functions in aneural activation of muscle-specific receptor kinase, which is required for postsynaptic differentiation, and in the subsequent clustering of the acetylcholine receptor in myotubes. This protein can also induce autophosphorylation of muscle-specific receptor kinase. Mutations in this gene are a cause of familial limb-girdle myasthenia autosomal recessive, which is also known as congenital myasthenic syndrome type 1B. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]

Uniprot Description

DOK7: docking proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. Probable muscle-intrinsic activator of MUSK that plays an essential role in neuromuscular synaptogenesis. Acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. Induces autophosphorylation of MUSK. Interacts with the cytoplasmic part of MUSK. Defects in DOK7 are the cause of congenital myasthenic syndrome type 1B or CMS1B. Three isoforms of the human protein are produced by alternative splicing.

Protein type: Activator; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: plasma membrane; cell junction; neuromuscular junction

Molecular Function: protein kinase binding; phosphoinositide binding; insulin receptor binding

Biological Process: receptor clustering; neuromuscular junction development

Disease: Myasthenia, Limb-girdle, Familial; Fetal Akinesia Deformation Sequence