Western Blot (WB)
(WB Suggested Anti-MDFIC Antibody Titration: 0.2-1 ug/mlPositive Control: MCF7 cell lysate)
Rabbit MDFIC Polyclonal Antibody | anti-MDFIC antibody
MDFIC antibody - N-terminal region
Western Blot (WB)
(WB Suggested Anti-MDFIC Antibody Titration: 0.2-1 ug/mlPositive Control: MCF7 cell lysate)
Western Blot (WB)
(WB Suggested Anti-MDFIC Antibody Titration: 0.2-1 ug/mlPositive Control: MCF7 cell lysate)
Target Description: MDFIC is a member of a family of proteins characterized by a specific cysteine-rich C-terminal domain, which is involved in transcriptional regulation of viral genome expression. Alternative translation initiation from an upstream non-AUG (GUG), and an in-frame, downstream AUG codon, results in the production of two isoforms, p40 and p32, respectively, which have different subcellular localization; p32 is mainly found in the cytoplasm, whereas p40 is targeted to the nucleolus. Both isoforms have transcriptional regulatory activity that is attributable to the cysteine-rich C-terminal domain.This gene product is a member of a family of proteins characterized by a specific cysteine-rich C-terminal domain, which is involved in transcriptional regulation of viral genome expression. Alternative translation initiation from an upstream non-AUG (GUG), and an in-frame, downstream AUG codon, results in the production of two isoforms, p40 and p32, respectively, which have different subcellular localization; p32 is mainly found in the cytoplasm, whereas p40 is targeted to the nucleolus. Both isoforms have transcriptional regulatory activity that is attributable to the cysteine-rich C-terminal domain.
NCBI and Uniprot Product Information
NCBI Description
This gene product is a member of a family of proteins characterized by a specific cysteine-rich C-terminal domain, which is involved in transcriptional regulation of viral genome expression. Alternative translation initiation from an upstream non-AUG (GUG), and an in-frame, downstream AUG codon, results in the production of two isoforms, p40 and p32, respectively, which have different subcellular localization; p32 is mainly found in the cytoplasm, whereas p40 is targeted to the nucleolus. Both isoforms have transcriptional regulatory activity that is attributable to the cysteine-rich C-terminal domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
Uniprot Description
MDFIC: Acts as a transcriptional activator or repressor. Inhibits the transcriptional activation of Zic family proteins ZIC1, ZIC2 and ZIC3. Retains nuclear Zic proteins ZIC1, ZIC2 and ZIC3 in the cytoplasm. Modulates the expression from both cellular and viral promoters. Down-regulates Tat-dependent transcription of the human immunodeficiency virus type 1 (HIV-1) LTR by interacting with HIV-1 Tat and Rev and impairing their nuclear import, probably by rendering the NLS domains inaccessible to importin- beta. Also stimulates activation of human T-cell leukemia virus type I (HTLV-I) LTR. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways. Belongs to the MDFI family. 2 isoforms of the human protein are produced by alternative initiation.
Protein type: Nucleolus
Chromosomal Location of Human Ortholog: 7q31.1-q31.2
Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; nucleolus; nucleus
Molecular Function: cyclin binding; protein binding; Tat protein binding; transcription factor binding
Biological Process: transcription, DNA-dependent; viral reproduction; positive regulation of viral transcription; positive regulation of transcription, DNA-dependent; regulation of Wnt receptor signaling pathway; negative regulation of protein import into nucleus; negative regulation of transcription, DNA-dependent; activation of JNK activity
Research Articles on MDFIC
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Product Notes
The MDFIC mdfic (Catalog #AAA3210139) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MDFIC antibody - N-terminal region reacts with Human, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's MDFIC can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the MDFIC mdfic for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: SVRPAGRRAR RQRRGAGSAE RPMSGAGEAL APGPVGPQRV AEAGGGQLGS. It is sometimes possible for the material contained within the vial of "MDFIC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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