Monkey Carnitine palmitoyltransferase I ELISA Kit | CPT-1 elisa kit

Monkey Carnitine palmitoyltransferase I ELISA Kit

Cat. Num. AAA750853

• Gene Names: Cpt1a; CPT-Ia
• Reactivity: Monkey
• Synonyms: Carnitine palmitoyltransferase I; Monkey Carnitine palmitoyltransferase I ELISA Kit; CPT-1 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This CPT-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey CPT-1. This ELISA kit for research use only, not for therapeutic applications
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CPT-1 elisa kit

Principle of the Assay: CPT-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CPT-1 antibody and an CPT-1-HRP conjugate. The assay sample and buffer are incubated together with CPT-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPT-1 concentration since CPT-1 from samples and CPT-1-HRP conjugate compete for the anti-CPT-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CPT-1 from the sample, fewer sites are left to bind CPT-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CPT-1 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 146345405
• NCBI GeneID: 25757
• Molecular Weight: 88,125 Da
• NCBI Official Full Name: Carnitine O-palmitoyltransferase 1, liver isoform
• NCBI Official Synonym Full Names: carnitine palmitoyltransferase 1a, liver
• NCBI Official Symbol: Cpt1a
• NCBI Official Synonym Symbols: CPT-Ia
• NCBI Protein Information: carnitine O-palmitoyltransferase 1, liver isoform; CPT I; CPT1-L; CPTI-L; carnitine palmitoyltransferase 1, liver; carnitine O-palmitoyltransferase I, liver isoform; Carnitine palmitoyltransferase 1 alpha, liver isoform
• UniProt Protein Name: Carnitine O-palmitoyltransferase 1, liver isoform
• UniProt Gene Name: Cpt1a
• UniProt Synonym Gene Names: Cpt-1; Cpt1; CPT1-L; CPT I; CPTI-L
• UniProt Entry Name: CPT1A_RAT

NCBI Description

enzyme that catalyses the transfer of long chain fatty acids to carnitine for translocation across the mitochondrial inner membrane [RGD, Feb 2006]

Uniprot Description

CPT1A: Belongs to the carnitine/choline acetyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Lipid Metabolism - fatty acid; Transferase; Membrane protein, multi-pass; Membrane protein, integral; EC 2.3.1.21

Cellular Component: endoplasmic reticulum membrane; mitochondrial outer membrane; membrane; mitochondrion; intracellular membrane-bound organelle; mitochondrial inner membrane; integral to membrane; integral to mitochondrial outer membrane

Molecular Function: identical protein binding; protein binding; carnitine O-palmitoyltransferase activity

Biological Process: response to drug; eating behavior; glucose metabolic process; long-chain fatty acid metabolic process; response to organic cyclic substance; positive regulation of fatty acid beta-oxidation; fatty acid beta-oxidation; triacylglycerol metabolic process; epithelial cell differentiation; transport; regulation of fatty acid oxidation; regulation of fatty acid beta-oxidation; carnitine metabolic process; regulation of insulin secretion; protein homooligomerization