Human Protein Kinase, AMP Activated Beta 1 (PRKAb1) RTU ELISA Kit | PRKAb1 rtu elisa kit

Human Protein Kinase, AMP Activated Beta 1 (PRKAb1) ELISA Kit

Cat. Num. AAA4502023

• Gene Names: PRKAB1; AMPK; HAMPKb
• Reactivity: Human
• Synonyms: Protein Kinase; AMP Activated Beta 1 (PRKAb1); Human Protein Kinase; AMP Activated Beta 1 (PRKAb1) ELISA Kit; AMPK; HAMPKb; 5'-AMP-Activated Protein Kinase Subunit Beta-1 Non-Catalytic Subunit; Adenosine Monophosphate Activated Protein Kinase(AMPK); PRKAb1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 270

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.124ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRKAb1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PRKAb1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PRKAb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PRKAb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PRKAb1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 19923359
• NCBI GeneID: 5564
• NCBI Accession #: NP_006244.2
• NCBI GenBank Nucleotide #: NM_006253.4
• UniProt Accession #: Q9Y478; Q9UBV0; Q9UE20; Q9UEX2; Q9Y6V8
• Molecular Weight: 30,382 Da
• NCBI Official Full Name: 5'-AMP-activated protein kinase subunit beta-1
• NCBI Official Synonym Full Names: protein kinase AMP-activated non-catalytic subunit beta 1
• NCBI Official Symbol: PRKAB1
• NCBI Official Synonym Symbols: AMPK; HAMPKb
• NCBI Protein Information: 5'-AMP-activated protein kinase subunit beta-1
• UniProt Protein Name: 5'-AMP-activated protein kinase subunit beta-1
• Protein Family: 5'-AMP-activated protein kinase
• UniProt Gene Name: PRKAB1
• UniProt Synonym Gene Names: AMPK; AMPK subunit beta-1; AMPKb

NCBI Description

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq, Jul 2008]

Uniprot Description

Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).