Typical Testing Data/Standard Curve (for reference only)
Mouse Carnitine palmitoyltransferase I ELISA Kit | CPT-1 elisa kit
Mouse Carnitine palmitoyltransferase I ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CPTI ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CPTI antibody and an CPTI-HRP conjugate. The assay sample and buffer are incubated together with CPTI-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPTI concentration since CPTI from samples and CPTI-HRP conjugate compete for the anti-CPTI antibody binding site. Since the number of sites is limited, as more sites are occupied by CPTI from the sample, fewer sites are left to bind CPTI-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CPTI concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
enzyme that catalyses the transfer of long chain fatty acids to carnitine for translocation across the mitochondrial inner membrane [RGD, Feb 2006]
Uniprot Description
CPT1A: Belongs to the carnitine/choline acetyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transferase; Lipid Metabolism - fatty acid; Mitochondrial; Membrane protein, multi-pass; Membrane protein, integral; EC 2.3.1.21
Cellular Component: mitochondrial outer membrane; endoplasmic reticulum membrane; intracellular membrane-bound organelle; membrane; mitochondrion; mitochondrial inner membrane; integral to membrane; integral to mitochondrial outer membrane
Molecular Function: identical protein binding; protein binding; carnitine O-palmitoyltransferase activity
Biological Process: response to drug; eating behavior; glucose metabolic process; long-chain fatty acid metabolic process; response to organic cyclic substance; positive regulation of fatty acid beta-oxidation; fatty acid beta-oxidation; triacylglycerol metabolic process; epithelial cell differentiation; transport; regulation of fatty acid oxidation; regulation of fatty acid beta-oxidation; carnitine metabolic process; regulation of insulin secretion; protein homooligomerization
Research Articles on CPT-1
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Product Notes
The Mouse CPT-1 cpt1a (Catalog #AAA723506) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA723506 ELISA Kit recognizes Mouse CPT-1. It is sometimes possible for the material contained within the vial of "Carnitine palmitoyltransferase I, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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