Specific Activity
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COVID 19 NSP10/NSP16 Methyltransferase Coronavirus Active Protein | COVID-19 active protein
2019-nCoV NSP10/NSP16 Methyltransferase, Active
Synonyms
COVID 19 NSP10/NSP16 Methyltransferase Coronavirus; 2019-nCoV NSP10/NSP16 Methyltransferase; Active; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; NSP10/NSP16 Methyltransferase; COVID-19 active protein
Host
E Coli
Form/Format
50mM sodium phosphate, pH7.0, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25% glycerol
Species
SARS-CoV-2
Tag
HIS
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Specific Activity
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Specific Activity
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Purity Data
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Purity Data
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Related Product Information for COVID-19 active protein
Recombinant 2019-nCoV NSP10/NSP16 methyltransferase includes full length NSP10 (A4254-Q4392) and NSP16 (S6799-N7096) expressed in E Coli using C-terminal His tag.
Overview: Many eukaryotic viruses have evolved 2'-O-methyltransferases (2'-O-MTase) to modify their viral mRNAs and carry a cap-1 structure (m7GpppNm) at the 5' end. This 5' cap structure is important for viral mRNA stability, protein translation and viral immune escape (1). SARS-CoV possess NSP16 that has 2'-O-MTase activity. NSP16 requires NSP 10 for activation which is a conserved mechanism in corona viruses (2). Therefore, inhibitors targeting the NSP10/NSP16 2'-O-MTase are potential targets for developing anti-Corona virus drugs (3).
Overview: Many eukaryotic viruses have evolved 2'-O-methyltransferases (2'-O-MTase) to modify their viral mRNAs and carry a cap-1 structure (m7GpppNm) at the 5' end. This 5' cap structure is important for viral mRNA stability, protein translation and viral immune escape (1). SARS-CoV possess NSP16 that has 2'-O-MTase activity. NSP16 requires NSP 10 for activation which is a conserved mechanism in corona viruses (2). Therefore, inhibitors targeting the NSP10/NSP16 2'-O-MTase are potential targets for developing anti-Corona virus drugs (3).
Product Categories/Family for COVID-19 active protein
References
1. Furuichi, Y. et al: Viral and cellular mRNA capping: past and prospects. Adv Virus Res. 2000, 55:135-184.
2. Decroly, E. et al. Corona virus nonstructural protein 16 is a cap-o binding enzyme possessing (nucleoside-2'O)-methyltransferase activity. J Virol. 2008, 82(16):8071-8084.
3. Chen, Y. et al: Biochemical and structural insights into the mechanisms of SARS Corona virus RNA ribose 2'-O-methylation by nsp16/nsp10 protein complex. PLoS Pathog. 2011, 7(10): e1002294.
2. Decroly, E. et al. Corona virus nonstructural protein 16 is a cap-o binding enzyme possessing (nucleoside-2'O)-methyltransferase activity. J Virol. 2008, 82(16):8071-8084.
3. Chen, Y. et al: Biochemical and structural insights into the mechanisms of SARS Corona virus RNA ribose 2'-O-methylation by nsp16/nsp10 protein complex. PLoS Pathog. 2011, 7(10): e1002294.
NCBI and Uniprot Product Information
Molecular Weight
38kDa (NSP16); 19kDa (NSP10)
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Product Notes
The COVID-19 (Catalog #AAA515761) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "COVID 19 NSP10/NSP16 Methyltransferase Coronavirus, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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