Testing Data
COVID 19 Methyltransferase Coronavirus Recombinant Protein | COVID-19 recombinant protein
SARS-CoV-2 (2019-nCoV) Methyltransferase/ME-his Recombinant Protein
Purity
>85 % as determined by SDS-PAGE.
Synonyms
COVID 19 Methyltransferase Coronavirus; SARS-CoV-2 (2019-nCoV) Methyltransferase/ME-his Recombinant Protein; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2COVID 19 Methyltransferase Coronavirus; Methyltransferase; COVID-19 recombinant protein
Host
E Coli
Purity/Purification
>85 % as determined by SDS-PAGE.
Form/Format
Liquid. Supplied as sterile 10mM Tris 250mM NaCl, 50% Glycerol pH7.4
Sequence Positions
299aa
Sequence
Ser6799-Asn7096
Species
2019-nCoV
Tag
N-His
Predicted N Terminal
Met
Protein Construction
|A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Methyltransferase/ME (YP_009724389.1) (Ser6799-Asn7096) was expressed with a polyhistidine tag at the C-terminus.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping: Ice pack
Shipping: Ice pack
Testing Data
Testing Data
Related Product Information for COVID-19 recombinant protein
Coronavirus encodes the 2'-O-MTase (2'O Methyltransferase) that is composed of the catalytic subunit nsp16 and the stimulatory subunit nsp10 and plays an important role in virus genome replication and evasion from innate immunity during viral infection. Nonstructural protein 16 (NSP16)/viral 2'O-methyltransferase (2'O-MTase) is highly conserved. The conserved 2'O-MTase activity is important for CoV pathogenesis and NSP16 is a conserved universal target for rapid live attenuated vaccine design in an expanding Coronavirus outbreak setting, such as COVID-19. Targeting the 2'O-methylation pathway on SARS-CoV replication and pathogenesis can be the treatment options for vaccine and anti-viral drug development which can against SARS-CoV-2, SARS-CoV, MERS-CoV or other RNA and DNA viruses.
References
Wang Y, et al., Coronavirus nsp10/nsp16 Methyltransferase Can Be Targeted by nsp10-Derived Peptide In Vitro and In Vivo To Reduce Replication and Pathogenesis.J Virol. 2015
Yu Chen, et al., Biochemical and Structural Insights into the Mechanisms of SARS Coronavirus RNA Ribose 2'-O-Methylation by nsp16/nsp10 Protein Complex.PLOS Pathogens.2011
Mickael Bouvet, et al., Coronavirus Nsp10: a Critical Co-Factor for Activation of Multiple Replicative Enzymes.JBC.2014
Yu Chen, et al., Biochemical and Structural Insights into the Mechanisms of SARS Coronavirus RNA Ribose 2'-O-Methylation by nsp16/nsp10 Protein Complex.PLOS Pathogens.2011
Mickael Bouvet, et al., Coronavirus Nsp10: a Critical Co-Factor for Activation of Multiple Replicative Enzymes.JBC.2014
NCBI and Uniprot Product Information
Molecular Weight
The recombinant SARS-CoV-2 (2019-nCoV) Methyltransferase/ME consists of 299 amino acids and predicts a molecular mass of 33.46kDa.
Similar Products
Product Notes
The COVID-19 (Catalog #AAA8118822) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 299aa. The amino acid sequence is listed below: Ser6799-As n7096. It is sometimes possible for the material contained within the vial of "COVID 19 Methyltransferase Coronavirus, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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