Bovine Vascular Endothelial cell Growth Factor B ELISA Kit | VEGF-B elisa kit

Bovine Vascular Endothelial cell Growth Factor B ELISA Kit

Cat. Num. AAA738436

• Gene Names: VEGFB; VRF; VEGFL
• Reactivity: Bovine
• Synonyms: Vascular Endothelial cell Growth Factor B; Bovine Vascular Endothelial cell Growth Factor B ELISA Kit; VEGF-B elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This VEGF-B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine VEGF-B. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VEGF-B elisa kit

Principle of the Assay: VEGF-B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-VEGF-B antibody and an VEGF-B-HRP conjugate. The assay sample and buffer are incubated together with VEGF-B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VEGF-B concentration since VEGF-B from samples and VEGF-B-HRP conjugate compete for the anti-VEGF-B antibody binding site. Since the number of sites is limited, as more sites are occupied by VEGF-B from the sample, fewer sites are left to bind VEGF-B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VEGF-B concentration in each sample is interpolated from this standard curve.

Product Categories/Family for VEGF-B elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 4507887
• NCBI GeneID: 7423
• NCBI Accession #: NP_003368.1
• NCBI GenBank Nucleotide #: NM_003377.4
• Molecular Weight: 21,261 Da
• NCBI Official Full Name: vascular endothelial growth factor B isoform VEGFB-186
• NCBI Official Synonym Full Names: vascular endothelial growth factor B
• NCBI Official Symbol: VEGFB
• NCBI Official Synonym Symbols: VRF; VEGFL
• NCBI Protein Information: vascular endothelial growth factor B; VEGF-related factor
• UniProt Protein Name: Vascular endothelial growth factor B
• UniProt Gene Name: VEGFB
• UniProt Synonym Gene Names: VRF; VEGF-B; VRF
• UniProt Entry Name: VEGFB_HUMAN

NCBI Description

This gene encodes a member of the PDGF (platelet-derived growth factor)/VEGF (vascular endothelial growth factor) family. The VEGF family members regulate the formation of blood vessels and are involved in endothelial cell physiology. This member is a ligand for VEGFR-1 (vascular endothelial growth factor receptor 1) and NRP-1 (neuropilin-1). Studies in mice showed that this gene was co-expressed with nuclear-encoded mitochondrial genes and the encoded protein specifically controlled endothelial uptake of fatty acids. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Sep 2011]

Uniprot Description

VEGFB: Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. Homodimer; disulfide-linked. Can also form heterodimer with VEGF. Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Secreted; Cell cycle regulation; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: extracellular space; membrane; extracellular region

Molecular Function: heparin binding; protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; vascular endothelial growth factor receptor 1 binding; chemoattractant activity

Biological Process: response to drug; platelet activation; protein amino acid O-linked glycosylation; positive regulation of vascular permeability; positive regulation of vascular endothelial growth factor receptor signaling pathway; induction of positive chemotaxis; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; platelet degranulation; positive chemotaxis; positive regulation of cell division; positive regulation of endothelial cell proliferation; negative regulation of neuron apoptosis; angiogenesis; vascular endothelial growth factor receptor signaling pathway; blood coagulation; negative regulation of apoptosis; cardiac muscle contraction