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Mouse Cortistatin ELISA Kit | CORT elisa kit

Mouse Cortistatin (CORT) ELISA Kit

Gene Names
Cort; CST; PCST
Reactivity
Mouse
Synonyms
Cortistatin; Mouse Cortistatin (CORT) ELISA Kit; COT; CROT; Peroxisomal Carnitine O-Octanoyltransferase; CORT elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse CORT. No significant cross-reactivity or interference between Mouse CORT and analogues was observed.
Sequence Length
112
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for CORT elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CORT in samples. An antibody specific for CORT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCORT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CORT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CORT bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
11,613 Da
NCBI Official Full Name
cortistatin preproprotein
NCBI Official Synonym Full Names
cortistatin
NCBI Official Symbol
Cort
NCBI Official Synonym Symbols
CST; PCST
NCBI Protein Information
cortistatin
UniProt Protein Name
Cortistatin
Protein Family
UniProt Gene Name
Cort

NCBI Description

This gene encodes a member of the somatostatin family of multifunctional peptides attributed with neurohormone, neurotransmitter/modulator and autocrine/paracrine actions. The encoded preproprotein undergoes proteolytic processing to generate a mature functional peptide that can bind to somatostatin receptors. Mice lacking the encoded protein exhibit elevated levels of growth hormone in the plasma without major changes in somatic growth and have exacerbated nociceptive responses to neuropathic and inflammatory pain sensitization. Transgenic mice overexpressing the encoded protein in neurons do not express long-term potentiation in the dentate gyrus and exhibit deficits in synaptic plasticity and learning. [provided by RefSeq, Nov 2015]

Uniprot Description

CORT: Binds to all human somatostatin receptor (SSTR) subtypes. It also inhibits cAMP production induced by forskolin through SSTRs. Belongs to the somatostatin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4 E2|4 78.89 cM

Cellular Component: extracellular region

Molecular Function: G-protein coupled receptor binding; hormone activity; neuropeptide hormone activity

Biological Process: G-protein signaling, adenylate cyclase inhibiting pathway; neuropeptide signaling pathway; regulation of circadian sleep/wake cycle, sleep

Research Articles on CORT

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Product Notes

The Mouse CORT cort (Catalog #AAA285988) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA285988 ELISA Kit recognizes Mouse CORT. It is sometimes possible for the material contained within the vial of "Cortistatin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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