5-Demethoxyubiquinone Hydroxylase Recombinant Protein | COQ7 recombinant protein

Recombinant Human 5-Demethoxyubiquinone Hydroxylase, Mitochondrial Protein

Cat. Num. AAA2556642

• Gene Names: COQ7; CAT5; CLK1; CLK-1; COQ10D8
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: 5-Demethoxyubiquinone Hydroxylase; Recombinant Human 5-Demethoxyubiquinone Hydroxylase; Mitochondrial Protein; Ubiquinone Biosynthesis Protein COQ7 Homolog; Coenzyme Q Biosynthesis Protein 7 Homolog; Timing Protein Clk-1 Homolog; COQ7; COQ7 recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Sequence Length: 179

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fragment: Ser37-Leu217
• Fusion Tag: C-6His
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days.; Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for COQ7 recombinant protein

Protein Construction: Recombinant Human 5-demethoxyubiquinone hydroxylase, mitochondrial is produced by our Mammalian expression system and the target gene encoding Ser37-Leu217 is expressed with a 6His tag at the C-terminus.

Background: Ubiquinone Biosynthesis Protein COQ7 Homolog (COQ7) is a mitochondrion inner membrane and peripheral membrane protein which belongs to the COQ7 family. It is expressed dominantly in heart and skeletal muscle. COQ7 is synthesized as a preprotein that is imported into the mitochondrial matrix, where the sequence is cleaved off and the mature protein becomes loosely associated with the inner membrane. COQ7 is involved in lifespan determination in ubiquinone-independent manner and also involved in ubiquinone biosynthesis. COQ7 is potential central metabolic regulator. Human COQ7 protein contains 179 amino acids, is mostly helical, and contains an alpha-helical membrane insertion. It has been shown that mutations in the gene are associated with increased life span. Defects of the gene slow down a variety of developmental and physiological processes, including the cell cycle, embryogenesis, post-embryonic growth, rhythmic behaviors and aging.

NCBI and Uniprot Product Information

• NCBI GI #: 300244542
• NCBI GeneID: 10229
• NCBI Accession #: NP_001177912.1
• NCBI GenBank Nucleotide #: NM_001190983.1
• UniProt Accession #: Q99807; Q9BTT7; Q9H0T5; Q9UEW5; Q9UNR5; B2RDA9
• Molecular Weight: Molecular Mass: 21.3kDa; Actual Protein Molecular Mass: 19-22kDa
• NCBI Official Full Name: 5-demethoxyubiquinone hydroxylase, mitochondrial isoform 2
• NCBI Official Synonym Full Names: coenzyme Q7, hydroxylase
• NCBI Official Symbol: COQ7
• NCBI Official Synonym Symbols: CAT5; CLK1; CLK-1; COQ10D8
• NCBI Protein Information: 5-demethoxyubiquinone hydroxylase, mitochondrial
• UniProt Protein Name: 5-demethoxyubiquinone hydroxylase, mitochondrial
• Protein Family: 5-demethoxyubiquinone hydroxylase
• UniProt Gene Name: COQ7
• UniProt Synonym Gene Names: DMQ hydroxylase

NCBI Description

The protein encoded by this gene is similar to a mitochondrial di-iron containing hydroxylase in Saccharomyces cerevisiae that is involved with ubiquinone biosynthesis. Mutations in the yeast gene lead to slower development and longer life span. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

Catalyzes the hydroxylation of 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) during ubiquinone biosynthesis. Has also a structural role in the COQ enzyme complex, stabilizing other COQ polypeptides. Involved in lifespan determination in a ubiquinone-independent manner.