COQ7 recombinant protein

COQ7 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121119

• Gene Names: COQ7; CAT5; CLK1; CLK-1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: COQ7; COQ7 Protein; Human; Recombinant (His Tag); Human COQ7 Protein (His Tag); CAT5 Protein; CLK-1 Protein; CLK1 Protein; coenzyme Q7 homolog; ubiquinone (yeast); COQ7 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ser37-Leu217

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ser 37
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mature form of human COQ7 isoform 1 (Q99807-1)(Ser 37-Leu 217) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for COQ7 recombinant protein

Background: Ubiquinone biosynthesis protein COQ7 homolog, also known as Coenzyme Q biosynthesis protein 7 homolog, Timing protein clk-1 homolog and COQ7, is a mitochondrion inner membrane and peripheral membrane protein which belongs to theCOQ7 family. It is expressed dominantly in heart and skeletal muscle. COQ7 is synthesized as a preprotein that is imported into the mitochondrial matrix, where the sequence is cleaved off and the mature protein becomes loosely associated with the inner membrane. This enzyme is responsible for the hydroxylation of 5-demethoxyubiquinone to 5-hydroxyubiquinone. Human COQ7 protein is mostly helical, and contains an alpha-helical membrane insertion. It has a potential N-glycosylation site, a phosphorylation site for protein kinase C and another for casein kinase II, and three N-myristoylation sites. COQ7 is involved in lifespan determination in ubiquinone-independent manner. It is also involved in ubiquinone biosynthesis. COQ7 is potential central metabolic regulator.

References

Ewbank J.J., et al., 1997, Science. 275: 980-3. Vajo Z., et al., 1999, Mamm. Genome. 10: 1000-4. Wiemann S., et al., 2001, Genome Res. 11: 422-35. Stenmark,P. et al., 2001, J Biol Chem. 276 (36): 33297-300. Martin J., et al., 2004, Nature. 432: 988-94.

NCBI and Uniprot Product Information

• NCBI GI #: 311033465
• NCBI GeneID: 10229
• UniProt Accession #: Q99807; Q9BTT7; Q9H0T5; Q9UEW5; Q9UNR5; B2RDA9
• Molecular Weight: 217
• NCBI Official Full Name: Ubiquinone biosynthesis protein COQ7 homolog
• NCBI Official Synonym Full Names: coenzyme Q7 homolog, ubiquinone (yeast)
• NCBI Official Symbol: COQ7
• NCBI Official Synonym Symbols: CAT5; CLK1; CLK-1
• NCBI Protein Information: ubiquinone biosynthesis protein COQ7 homolog; placental protein KG-20; timing protein clk-1 homolog; COQ7 coenzyme Q, 7 homolog ubiquinone; coenzyme Q biosynthesis protein 7 homolog
• UniProt Protein Name: Ubiquinone biosynthesis protein COQ7 homolog
• Protein Family: 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase
• UniProt Gene Name: COQ7
• UniProt Entry Name: COQ7_HUMAN

NCBI Description

The protein encoded by this gene is similar to a mitochondrial di-iron containing hydroxylase in Saccharomyces cerevisiae that is involved with ubiquinone biosynthesis. Mutations in the yeast gene lead to slower development and longer life span. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

COQ7: Involved in lifespan determination in ubiquinone- independent manner. Involved in ubiquinone biosynthesis. Potential central metabolic regulator. Belongs to the COQ7 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cofactor and Vitamin Metabolism - ubiquinone and other terpenoid-quinone biosynthesis; Mitochondrial

Chromosomal Location of Human Ortholog: 16p12.3

Cellular Component: mitochondrial inner membrane; nucleus

Molecular Function: metal ion binding

Biological Process: ubiquinone biosynthetic process; neurogenesis; in utero embryonic development; determination of adult life span; neural tube formation; age-dependent response to oxidative stress; organelle ATP synthesis coupled electron transport