Western Blot (WB)
(Western blot analysis of extracts of mouse heart tissue, using COQ7 antibody.)
Rabbit COQ7 Polyclonal Antibody | anti-COQ7 antibody
COQ7 Antibody
Gene Names
COQ7; CAT5; CLK1; CLK-1; COQ10D8
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
COQ7; Polyclonal Antibody; COQ7 Antibody; 5 demethoxyubiquinone hydroxylase mitochondrial; CAT5; CLK 1; CLK1; Coenzyme Q biosynthesis protein 7 homolog; Coenzyme Q7 homolog ubiquinone; Coenzyme Q7 hydroxylase; COQ10D8; coq7; COQ7 coenzyme Q 7 homolog ubiquinone; COQ7 protein; COQ7_HUMAN; DMQ hydroxylase; Placental protein KG 20; Timing protein clk-1 homolog; Ubiquinone biosynthesis monooxygenase COQ7; Ubiquinone biosynthesis protein COQ7 homolog; anti-COQ7 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
COQ7 antibody detects endogenous levels of COQ7.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
179
Applicable Applications for anti-COQ7 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human COQ7.
Subcellular Location
Mitochondrion Inner Membrane. Synthesized as a preprotein that is imported into the mitochondrial matrix, where the sequence is cleaved off and the mature protein becomes loosely associated with the inner membrane.
Tissue Specificity
Expressed dominantly in heart and skeletal muscle.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts of mouse heart tissue, using COQ7 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of mouse heart tissue, using COQ7 antibody.)
Immunohistochemistry (IHC)
(MBS9603487 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603487 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-COQ7 antibody
Function: Catalyzes the hydroxylation of 2-polyprenyl-3-methyl-6-methoxy-1, 4-benzoquinol (DMQH2) during ubiquinone biosynthesis. Has also a structural role in the COQ enzyme complex, stabilizing other COQ polypeptides. Involved in lifespan determination in a ubiquinone-independent manner.
Subunit Structure: Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9 (By similarity). Interacts with COQ8B and COQ6 (PubMed:24270420). Interacts with COQ9 (PubMed:25339443).
Similarity: Belongs to the COQ7 family.
Subunit Structure: Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9 (By similarity). Interacts with COQ8B and COQ6 (PubMed:24270420). Interacts with COQ9 (PubMed:25339443).
Similarity: Belongs to the COQ7 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 22 kDa
Predicted: 25 kDa
Predicted: 25 kDa
NCBI Official Full Name
5-demethoxyubiquinone hydroxylase, mitochondrial isoform 2
NCBI Official Synonym Full Names
coenzyme Q7, hydroxylase
NCBI Official Symbol
COQ7
NCBI Official Synonym Symbols
CAT5; CLK1; CLK-1; COQ10D8
NCBI Protein Information
5-demethoxyubiquinone hydroxylase, mitochondrial
UniProt Protein Name
5-demethoxyubiquinone hydroxylase, mitochondrial
Protein Family
UniProt Gene Name
COQ7
UniProt Synonym Gene Names
DMQ hydroxylase
NCBI Description
The protein encoded by this gene is similar to a mitochondrial di-iron containing hydroxylase in Saccharomyces cerevisiae that is involved with ubiquinone biosynthesis. Mutations in the yeast gene lead to slower development and longer life span. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]
Uniprot Description
Catalyzes the hydroxylation of 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) during ubiquinone biosynthesis. Has also a structural role in the COQ enzyme complex, stabilizing other COQ polypeptides. Involved in lifespan determination in a ubiquinone-independent manner.
Research Articles on COQ7
Similar Products
Product Notes
The COQ7 coq7 (Catalog #AAA9603487) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The COQ7 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's COQ7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the COQ7 coq7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COQ7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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