Rat Thymosin Beta 4 ELISA Kit | TMSbeta4 elisa kit

Rat Thymosin Beta 4 ELISA Kit

Cat. Num. AAA761243

• Gene Names: TMSB4X; FX; TB4X; PTMB4; TMSB4
• Reactivity: Rat
• Synonyms: Thymosin Beta 4; Rat Thymosin Beta 4 ELISA Kit; TMSb4 ; TMSbeta4 elisa kit

• Reactivity: Rat
• Sequence Length: 44

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Competitive ELISA, Coated with Antigen
• Detection Range: 0.391-25ng/ml
• Sensitivity: 0.234ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for TMSbeta4 elisa kit

Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Rat TMSbeta4. During the reaction,Rat TMSbeta4 in the sample or standard competes with a fixed amount of Rat TMSbeta4 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Rat TMSbeta4. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat TMSbeta4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 11056061
• NCBI GeneID: 7114
• NCBI Accession #: NP_066932.1
• NCBI GenBank Nucleotide #: NM_021109.3
• Molecular Weight: 5,053 Da
• NCBI Official Full Name: thymosin beta-4
• NCBI Official Synonym Full Names: thymosin beta 4, X-linked
• NCBI Official Symbol: TMSB4X
• NCBI Official Synonym Symbols: FX; TB4X; PTMB4; TMSB4
• NCBI Protein Information: thymosin beta-4
• UniProt Protein Name: Thymosin beta-4
• UniProt Gene Name: TMSB4X
• UniProt Synonym Gene Names: TB4X; THYB4; TMSB4; T beta-4
• UniProt Entry Name: TYB4_HUMAN

NCBI Description

This gene encodes an actin sequestering protein which plays a role in regulation of actin polymerization. The protein is also involved in cell proliferation, migration, and differentiation. This gene escapes X inactivation and has a homolog on chromosome Y. [provided by RefSeq, Jul 2008]

Uniprot Description

TMSB4X: Plays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization. Belongs to the thymosin beta family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xq21.3-q22

Cellular Component: cytoskeleton; extracellular region

Molecular Function: actin monomer binding; protein binding

Biological Process: actin filament organization; blood coagulation; platelet activation; platelet degranulation