Selectin, Leukocyte (SELL) Recombinant Protein | SELL recombinant protein

Recombinant Selectin, Leukocyte (SELL)

Cat. Num. AAA2010364

• Gene Names: Sell; A.11; LECAM-1; L-selectin
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Selectin; Leukocyte (SELL); Recombinant Selectin; SELL recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF- IGKTWTW VGTNKTLTKE AENWGTGEPN NKKSKEDCVE IYIKRERDSG KWNDDACHKR KAALCYTASC QPESCNRHGE CVETINNNTC ICDPGYYGPQ CQYVIQCEPL KAPELGTMNC IHPLGDFSFQ SQCAFNCSEG SELLGNAKTE CGASGNWTYL EPICQVIQCM PLAAPDLGTM ECSHPLANFS FTSACTFTCS EETDLIGERK TVCRSSGSWS SPSPICQKTK RSFSKIKEGD YN
• Sequence Length: 372

• Applicable Applications for SELL recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Ile94~Asn332 (Accession # P30836) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 400181
• NCBI GeneID: 29259
• UniProt Accession #: P30836
• Molecular Weight: 28.0kDa
• NCBI Official Full Name: L-selectin
• NCBI Official Synonym Full Names: selectin L
• NCBI Official Symbol: Sell
• NCBI Official Synonym Symbols: A.11; LECAM-1; L-selectin
• NCBI Protein Information: L-selectin; LAM-1; ly-22; LECAM1; selectin, lymphocyte; lymphocyte antigen 22; lymph node homing receptor; leukocyte adhesion molecule 1; CD62 antigen-like family member L; lymphocyte surface MEL-14 antigen; leukocyte-endothelial cell adhesion molecule 1
• UniProt Protein Name: L-selectin
• Protein Family: L-selectin
• UniProt Gene Name: Sell
• UniProt Synonym Gene Names: Lnhr; Ly-22; LAM-1; LECAM1; Ly-22
• UniProt Entry Name: LYAM1_RAT

NCBI Description

lymphocyte adhesion molecule that facilitates adhesion of lymphocytes to high endothelial venules [RGD, Feb 2006]

Uniprot Description

Function: Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia

By similarity.

Subunit structure: Interaction with PSGL1/SELPLG and PODXL2 is required for promoting recruitment and rolling of leukocytes. This interaction is dependent on the sialyl Lewis X glycan modification of PSGL1 and PODXL2, and tyrosine sulfation modifications of PSGL1. Sulfation on 'Tyr-51' of PSGL1 is important for L-selectin binding

By similarity.

Subcellular location: Membrane; Single-pass type I membrane protein.

Tissue specificity: Expressed in peripheral blood mononuclear cells (PBMC), spleen and thymus. Ref.1

Induction: Down-regulated by mitogen stimulation of PBMC and spleen T-cells. Ref.1

Sequence similarities: Belongs to the selectin/LECAM family.Contains 1 C-type lectin domain.Contains 1 EGF-like domain.Contains 2 Sushi (CCP/SCR) domains.