Ephrin A4 Active Protein | EFNA4 active protein

Ephrin A4 Protein, Human, Recombinant (hFc Tag)

• Gene Names: EFNA4; EFL4; EPLG4; LERK4
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Ephrin A4; Ephrin A4 Protein; Human; Recombinant (hFc Tag); Human Ephrin-A4/EFNA4 Protein (Fc Tag); EFL4 Protein; EPLG4 Protein; LERK4 Protein; ephrin-A4; EFNA4 active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly171

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 26
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human EFNA4 (NP_005218.1)(Met1-Gly171) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1. Measured by its binding ability in a functional ELISA.; 2. Immobilized human EPHA7-His at 10ug/mL(100uL/well) can bind human EFNA4-Fc (NEW). The EC50 of EFNA4-Fc (NEW) is 2.6-6.1ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized human EPHA7-His at 10 ug/mL(100 uL/well) can bind human EFNA4-Fc (NEW). The EC50 of EFNA4-Fc (NEW) is 2.6-6.1 ng/mL.)

Related Product Information for EFNA4 active protein

Background: EPH-related receptor tyrosine kinase ligand 4 (Ephrin-A4) also known as EFNA4, is a member of the Ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. Ephrin-A4/EFNA4 functions as a cell surface GPI-bound ligand for Eph receptor, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development.

References

Aasheim HC, et al. (2000) A splice variant of human ephrin-A4 encodes a soluble molecule that is secreted by activated human B lymphocytes. Blood. 95(1): 221-30.Moss A, et al. (2005) Ephrin-A4 inhibits sensory neurite outgrowth and is regulated by neonatal skin wounding. Eur J Neurosci. 22(10): 2413-21.Cerretti DP, et al. (1998) Characterization of the genes for mouse LERK-3/Ephrin-A3 (Epl3), mouse LERK-4/Ephrin-A4 (Epl4), and human LERK-6/Ephrin-A2 (EPLG6): conservation of intron/exon structure. Genomics. 47(1): 131-5.

NCBI and Uniprot Product Information

• NCBI GI #: 4885197
• NCBI GeneID: 1945
• NCBI Accession #: NP_005218.1
• NCBI GenBank Nucleotide #: NM_005227.2
• UniProt Accession #: P52798; O95457; Q5SR71; Q6FI57; C9JHJ8
• Molecular Weight: 22,386 Da
• NCBI Official Full Name: ephrin-A4 isoform a
• NCBI Official Synonym Full Names: ephrin-A4
• NCBI Official Symbol: EFNA4
• NCBI Official Synonym Symbols: EFL4; EPLG4; LERK4
• NCBI Protein Information: ephrin-A4; LERK-4; ligand of eph-related kinase 4; eph-related receptor tyrosine kinase ligand 4
• UniProt Protein Name: Ephrin-A4
• Protein Family: Ephrin
• UniProt Gene Name: EFNA4
• UniProt Synonym Gene Names: EPLG4; LERK4; LERK-4
• UniProt Entry Name: EFNA4_HUMAN

NCBI Description

This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNA class ephrin. Three transcript variants that encode distinct proteins have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

EFNA4: Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. May play a role in the interaction between activated B-lymphocytes and dendritic cells in tonsils. Belongs to the ephrin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Ligand, receptor tyrosine kinase

Chromosomal Location of Human Ortholog: 1q21-q22

Cellular Component: integral to plasma membrane; plasma membrane; extracellular region

Molecular Function: protein binding; transmembrane-ephrin receptor activity; ephrin receptor binding

Biological Process: axon guidance; cell-cell signaling; ephrin receptor signaling pathway; osteoclast differentiation; bone remodeling