COMMD1 recombinant protein

COMMD1, 1-190aa, Human, His tag, E Coli

Cat. Num. AAA203629

• Gene Names: COMMD1; MURR1; C2orf5
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: COMMD1; 1-190aa; Human; His tag; E Coli; COMM domain-containing protein 1; C2orf5; MuRR1; COMMD-1; COMMD 1; COMM; MuRR 1; MuRR-1; COMMD1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol 0.1M NaCl
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MAAGELEGGK PLSGLLNALA QDTFHGYPGI TEELLRSQLY PEVPPEEFRP FLAKMRGILK SIASADMDFN QLEAFLTAQT KKQGGITSDQ AAVISKFWKS HKTKIRESLM NQSRWNSGLR GLSWRVDGKS QSRHSAQIHT PVAIIELELG KYGQESEFLC LEFDEVKVNQ ILKTLSEVEE SISTLISQPN
• Sequence Length: 190

• Applicable Applications for COMMD1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for COMMD1 recombinant protein

COMMD1, also known as copper metabolism MuRR1 domain-containing protein 1, is a 190 amino acid protein responsible for inhibition of TNF-induced NFkB p50 and has a suggested role in facilitation of biliary copper excretion within hepatocytes. High expression is found in liver tissue, with lower expressions in lung, heart, kidney and brain tissue. This protein interacts directly with COMMD6 and ATP7B, and indirectly with IkB-beta and COMMD7. Recombinant human COMMD1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for COMMD1 recombinant protein

Signal Transduction

References

Van De Sluis B, et al. (2002) Hum Mol Genet. 11 (2):165-73.; Muller T, et al. (2003) J Hepatol. 38 (2):164-8.;

NCBI and Uniprot Product Information

• NCBI GI #: 22749079
• NCBI GeneID: 150684
• NCBI Accession #: NP_689729
• NCBI GenBank Nucleotide #: NM_152516.3
• UniProt Accession #: Q8N668; Q96GS0; B4DFQ4
• Molecular Weight: 23.3 kDa (210aa), confirmed by MALDI-TOF
• NCBI Official Full Name: COMM domain-containing protein 1 isoform 1
• NCBI Official Synonym Full Names: copper metabolism domain containing 1
• NCBI Official Symbol: COMMD1
• NCBI Official Synonym Symbols: MURR1; C2orf5
• NCBI Protein Information: COMM domain-containing protein 1
• UniProt Protein Name: COMM domain-containing protein 1
• Protein Family: COMM domain-containing protein
• UniProt Gene Name: COMMD1
• UniProt Synonym Gene Names: C2orf5; MURR1

NCBI Description

COMMD1 is a regulator of copper homeostasis, sodium uptake, and NF-kappa-B (see MIM 164011) signaling (de Bie et al., 2005 [PubMed 16267171]).[supplied by OMIM, Sep 2009]

Uniprot Description

Proposed scaffold protein that is implicated in diverse physiological processes and whose function may be in part linked to its ability to regulate ubiquitination of specific cellular proteins. Can modulate activity of cullin-RING E3 ubiquitin ligase (CRL) complexes by displacing CAND1; in vitro promotes CRL E3 activity and dissociates CAND1 from CUL1 and CUL2 (PubMed:21778237). Promotes ubiquitination of NF-kappa-B subunit RELA and its subsequent proteasomal degradation. Down-regulates NF-kappa-B activity (PubMed:15799966, PubMed:17183367, PubMed:20048074). Involved in the regulation of membrane expression and ubiquitination of SLC12A2 (PubMed:23515529). Modulates Na+ transport in epithelial cells by regulation of apical cell surface expression of amiloride-sensitive sodium channel (ENaC) subunits and by promoting their ubiquitination presumably involving NEDD4L. Promotes the localization of SCNN1D to recycling endosomes (PubMed:14645214, PubMed:20237237, PubMed:21741370). Promotes CFTR cell surface expression through regulation of its ubiquitination (PubMed:21483833). Down-regulates SOD1 activity by interfering with its homodimerization (PubMed:20595380). Plays a role in copper ion homeostasis. Involved in copper-dependent ATP7A trafficking between the trans-Golgi network and vesicles in the cell periphery; the function is proposed to depend on its association within the CCC complex and cooperation with the WASH complex on early endosomes (PubMed:25355947). Can bind one copper ion per monomer (PubMed:17309234). May function to facilitate biliary copper excretion within hepatocytes. Binds to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) (PubMed:18940794). Involved in the regulation of HIF1A-mediated transcription; competes with ARNT/Hif-1-beta for binding to HIF1A resulting in decreased DNA binding and impaired transcriptional activation by HIF-1 (PubMed:20458141).